Gene Expression And Transcription | Research Area | St John's Laboratory

Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g EGFR antibody (STJ113769). Western blot was performed from the immunoprecipitate using EGFR antibody (STJ113769) at a dilution of 1:1000.

Anti-EGFR antibody (1021-1210) (STJ113769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A-431 cells using [KD Validated] EGFR Rabbit polyclonal antibody (STJ113840) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-EGFR antibody (1021-1210) (STJ113840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (STJ113701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EGFR antibody (1026-1126) (STJ113701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunocytochemistry analysis of EGFR (green) in 3T3 using EGFR antibody, and DAPI (blue).

Anti-EGFR antibody (1075-1093 aa) [R01-2D3] (STJA0012677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£264.50

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Immunocytochemistry analysis of EGFR (green) in Hela using EGFR antibody, and DAPI (blue)

Anti-EGFR antibody (1075-1093 aa) [R03-6B1] (STJA0013018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£264.50

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Anti-EGFR antibody (1075-1093 aa) [R09-7I5] (STJA0011260)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£246.50

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Western blot analysis of extracts of Rat brain, using EGFR antibody (STJ113559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-EGFR antibody (1100-1200) (STJ113559)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ERBB1 antibody at 1:2500 dilution 50 µg of total protein per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-EGFR antibody (1162aa C-Term) (STJ140129)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Human appendix tissue was stained with Anti-EGFR (PTR2121) Antibody

Anti-EGFR antibody (300-400) [PTR2121] (STJA0006243)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit monoclonal antibody (STJ11105117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EGFR antibody (320-420 aa) [S5117RM] (STJ11105117)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma stained with anti-EGFR antibody using peroxidase-conjugate and DAB chromogen. Note the membrane staining of carcinoma cells

Anti-EGFR antibody (344-492aa) [ZR16] (STJ180567)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£170.50 - £624.50

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Western blot analysis of lysates from wild type (WT) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit polyclonal antibody (STJ11104511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EGFR antibody (350-450) (STJ11104511)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various cells using EGFR Polyclonal Antibody diluted at 1：1000

Anti-EGFR antibody (661-710 aa) (STJ92850)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-EGFR antibody (800-900) [S9MR] (STJ11102229)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded Carcinoma of Human lung tissue using EGFR antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EGFR antibody (900-1210 aa) [1B10] (STJA0013541)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£292.50

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Immunofluorescence analysis of A431 cells using EGFR antibody (STJ113839) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EGFR antibody (960-1060) (STJ113839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of human-liver tissue. 1, EGFR Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-EGFR antibody (986-1035 aa) (STJ92862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-EGFR antibody (N-Term) (STJA0005395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-EGFR antibody [2+00E+09] (STJ16100028)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IP/EM

Reactivity :

Human

£253.50

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Western blot detection of EGFR in HCC827, A549 and MDA-MB-468 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.

Anti-EGFR antibody [3F12-1H7-A10] (STJ99228)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC/IP

Reactivity :

Human/Monkey

£144.00 - £348.00

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Western blot detection of EGFR in A549, MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.

Anti-EGFR antibody [5E10-G10-E11] (STJ99229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Monkey

£144.00 - £348.00

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Western blot analysis of 1) A431, 2) Hela, diluted at 1:2000.

Anti-EGFR antibody [M6] (STJ97036)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of EGFR at dilution of 1:1000

Anti-EGFR antibody [SM4913] (STJA0004913)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£170.50 - £233.50

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Western blot analysis of EGFR at dilution of 1:2000

Anti-EGFR antibody [SM4954] (STJA0004954)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£170.50 - £233.50

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Anti-EGFR antibody [SRM] (STJ11106993)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using EHMT1 antibody (STJ110811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EHMT1 antibody (1-260) (STJ110811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using EHMT1 monoclonal antibody against EHMT1 recombinant protein.

Anti-EHMT1 antibody [1B7B4] (STJ98015)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-EHMT1/GLP1 antibody (950-100) (STJ506053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£427.50

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Anti-EHMT1/GLP1 antibody (950-100) {Biotin} (STJ506054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-EHMT1/GLP1 antibody (950-100) {FITC} (STJ506055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Western blot analysis of extracts of A375 cells, using EHMT2 antibody (STJ11103018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EHMT2 antibody (2-300) [S8MR] (STJ11103018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-EHMT2 antibody (370-450 aa) (STJ192390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells, using EHMT2 Rabbit polyclonal antibody (STJ11104967). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-EHMT2 antibody (680-813) (STJ11104967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g [KO Validated] EHMT2 antibody (STJ23499). Western blot was performed from the immunoprecipitate using [KO Validated] EHMT2 antibody (STJ23499) at a dilution of 1:1000.

Anti-EHMT2 antibody (877-1176) (STJ23499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using eIF2C3 Rabbit monoclonal antibody (STJ11103159) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-eIF2C3 antibody (721-860) [S9MR] (STJ11103159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using eIF4A3 Rabbit polyclonal antibody (STJ23515) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF4A3 antibody (1-210) (STJ23515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EIF4A3 antibody (218-268 aa) (STJ194381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using ELAC2 antibody (STJ29208) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ELAC2 antibody (1-270) (STJ29208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using ELAC2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ELAC2 antibody (161-210 aa) (STJ95517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-ELF1 antibody (1-210) (STJ28984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of 293 cells using Elf-1 Polyclonal Antibody diluted at 1：1000

Anti-ELF1 antibody (537-586 aa) (STJ92888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using ELF2 antibody (STJ29623) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ELF2 antibody (1-140) (STJ29623)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ELF2 antibody (80-130 aa) (STJ194039)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ELL Rabbit polyclonal antibody (STJ118876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ELL antibody (350-450) (STJ118876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using ELL Rabbit polyclonal antibody (STJ118877) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ELL antibody (350-450) (STJ118877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of Rat testis, using ELL2 Rabbit polyclonal antibody (STJ119700) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ELL2 antibody (200-640) (STJ119700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ELL2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ELL2 antibody (448-497 aa) (STJ92896)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ELL3 antibody (210-290 aa) (STJ191345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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