Anti-CDK12 antibody (1-300) (STJ117094)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CDK12 (1-300) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CDK12 |
| Gene ID | 51755 |
| Uniprot ID | CDK12_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MPNSERHGGKKDGSGGASGT LQPSSGGGSSNSRERHRLVS KHKRHKSKHSKDMGLVTPEA ASLGTVIKPLVEYDDISSDS DTFSDDMAFKLDRRENDERR GSDRSDRLHKHRHHQHRRSR DLLKAKQTEKEKSQEVSSKS GSMKDRISGSSKRSNEETDD YGKAQVAKSSSKESRSSKLH KEKTRKERELKSGHKDRSKS HRKRETPKSYKTVDSPKRRS RSPHRKWSDSSKQDDSPSG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CDK12 (NP_057591.2). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Phosphorylation at Thr-893 increases kinase activity. |
| Function | Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors. |
| Protein Name | Cyclin-Dependent Kinase 12Cdc2-Related Kinase - Arginine/Serine-RichCrkrsCell Division Cycle 2-Related Protein Kinase 7Cdc2-Related Protein Kinase 7Cell Division Protein Kinase 12Hcdk12 |
| Database Links | Reactome: R-HSA-6796648 |
| Cellular Localisation | NucleusNucleus SpeckleColocalized With Nuclear Speckles Throughout Interphase |
| Alternative Antibody Names | Anti-Cyclin-Dependent Kinase 12 antibodyAnti-Cdc2-Related Kinase - Arginine/Serine-Rich antibodyAnti-Crkrs antibodyAnti-Cell Division Cycle 2-Related Protein Kinase 7 antibodyAnti-Cdc2-Related Protein Kinase 7 antibodyAnti-Cell Division Protein Kinase 12 antibodyAnti-Hcdk12 antibodyAnti-CDK12 antibodyAnti-CRK7 antibodyAnti-CRKRS antibodyAnti-KIAA0904 antibody |
Information sourced from Uniprot.org