Anti-CDC27 antibody [R04-8U-5] (STJA0033740)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPeptide
General Information
| Short Description | Rabbit monoclonal anti-CDC27 for use in WB, IHC-P, ICC, IF and FC in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/FC |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-8U-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Protein A |
| Dilution Range | WB 1:1000-1:5000IHC-P 1:200-1:1000ICC:IF 1:50FC 1:50-1:100 |
| Formulation | Liquid in PBS, Glycerol and BSA |
| Storage Instruction | Store at-20°C. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CDC27 |
| Gene ID | 996 |
| Uniprot ID | CDC27_HUMAN |
| Immunogen | Peptide |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis. |
| Function | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. |
| Protein Name | Cell Division Cycle Protein 27 HomologAnaphase-Promoting Complex Subunit 3Apc3Cdc27 HomologCdc27hsH-Nuc |
| Database Links | Reactome: R-HSA-141430Reactome: R-HSA-174048Reactome: R-HSA-174084Reactome: R-HSA-174154Reactome: R-HSA-174178Reactome: R-HSA-174184Reactome: R-HSA-176407Reactome: R-HSA-176408Reactome: R-HSA-176409Reactome: R-HSA-176412Reactome: R-HSA-179409Reactome: R-HSA-2467813Reactome: R-HSA-2559582Reactome: R-HSA-68867Reactome: R-HSA-69017Reactome: R-HSA-8853884Reactome: R-HSA-9687136Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle |
| Alternative Antibody Names | Anti-Cell Division Cycle Protein 27 Homolog antibodyAnti-Anaphase-Promoting Complex Subunit 3 antibodyAnti-Apc3 antibodyAnti-Cdc27 Homolog antibodyAnti-Cdc27hs antibodyAnti-H-Nuc antibodyAnti-CDC27 antibodyAnti-ANAPC3 antibodyAnti-D0S1430E antibodyAnti-D17S978E antibody |
Information sourced from Uniprot.org