Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various lysates using SET1A Rabbit polyclonal antibody (STJ11100188) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5min.

Anti-SETD1A antibody (258-458 aa) (STJ11100188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-SETD1A antibody (50-100 aa) (STJ13102252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£322.50

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Anti-SETD1A antibody (STJA0009766)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of various lysates using SETD1B Rabbit polyclonal antibody (STJ11103186) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-SETD1B antibody (1673-1770) (STJ11103186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SETD1B antibody (STJA0007705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and SETDB1 knockout (KO) 293T cells, using [KO Validated] SETDB1 Rabbit pAb (STJ27898) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-SETDB1 antibody (1-397) (STJ27898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis using ESET monoclonal antibody against MCF-7 (1) , T47D (2) , HEK293 (3) , JURKAT (4) , NIH/3T3 (5) , F9 (6) , RAW246.7 (7) and Cos7 (8) cell lysate.

Anti-SETDB1 antibody [5H6A12] (STJ98056)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£149.00 - £360.00

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Human adrenal gland stained with anti-SF-1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of cortical cells.

Anti-SF-1 antibody (200-400aa) [ZR397] (STJ180598)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£181.50 - £844.50

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Western blot analysis of various lysates using SF3B1 Rabbit mAb (STJ11103676) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-SF3B1 antibody (1-100) [S6MR] (STJ11103676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using SF3B1 antibody (STJ118246) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SF3B1 antibody (1-144) (STJ118246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts from K562 cells, using SAP 155 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-SF3B1 antibody (75-124 aa) (STJ96403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-SF3B1 antibody [R01-8T1] (STJA0036820)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-SF3B1 antibody [R08-6I2] (STJA0035360)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Hamster/Rat

£133.50 - £281.50

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Immunohistochemistry analysis of 14-3-3 sigma in paraffin-embedded mouse skin using 14-3-3 sigma Rabbit polyclonal antibody (STJ25500) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SFN antibody (1-100) (STJ25500)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human colon using 14-3-3 sigma Rabbit monoclonal antibody (STJ11102062) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-SFN antibody (1-100) [S2MR] (STJ11102062)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunohistochemistry of mouse lung with Anti-SFN primary antibody (STJ502960) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SFN antibody (150-220) (STJ502960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-SFN antibody (150-220) {Biotin} (STJ502961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-SFN antibody (150-220) {FITC} (STJ502962)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-SFN antibody (41-90 aa) (STJ91377)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-SGF29 antibody (20-70 aa) (STJ13102246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£322.50

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Anti-SGF29 antibody (STJA0008320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using SGK1 Rabbit monoclonal antibody (STJ11101969) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SGK1 antibody (1-100) [S9MR] (STJ11101969)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£126.50 - £554.50

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Immunofluorescence analysis of L929 cells using SGK1 Rabbit polyclonal antibody (STJ25513) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SGK1 antibody (172-431) (STJ25513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using SGK1 Mouse mAb diluted at 1:200.

Anti-SGK1 antibody (350-430 aa) [4D12] (STJ97721)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, SGK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Alpha-SMA monoclonal antibody (6A12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SGK1 antibody (381-430 aa) (STJ95633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry of rat tissue with Anti-SGK1 primary antibody (STJ502978) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 10X.

Anti-SGK1 antibody (40-90) (STJ502978)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£400.50

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Anti-SGK1 antibody (40-90) {Biotin} (STJ502979)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£497.50

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Anti-SGK1 antibody (40-90) {FITC} (STJ502980)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Rat

£497.50

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Anti-SGK1 antibody [6E4-8G5-6D10] (STJA0032892)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Anti-SGK1 antibody [PT1163R] (STJA0040008)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Anti-SGK1 antibody [R07-4G3] (STJA0035995)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-SHP2 antibody (N-Term) [M163] (STJA0041374)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£474.50

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Anti-SHP2 antibody [1F7] (STJA0032702)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC

Reactivity :

Human/Mouse/Rat

£224.50 - £308.50

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Anti-SHP2 antibody [R08-3G2] (STJA0033804)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£140.50 - £308.50

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Anti-SHP2 antibody [R09-4N1] (STJA0036082)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-SHP2 antibody [SIT-01] (STJ16100481)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP/WB

Reactivity :

Human

£504.50

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Anti-SHP2 antibody [SRM13228] (STJ11107958)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£0.00

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Western blot analysis of extracts of various cell lines, using Sin3A antibody (STJ11104619) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SIN3A antibody (1-100) [S4619RM] (STJ11104619)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using Sin3A antibody (STJ11104830) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SIN3A antibody (1-100) [S4830RM] (STJ11104830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of extracts of 293T cells, using Sin3A antibody (STJ115863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SIN3A antibody (1-120) (STJ115863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g Sin3A antibody (STJ25530). Western blot was performed from the immunoprecipitate using Sin3A antibody (STJ25530) at a dilution of 1:1000.

Anti-SIN3A antibody (1-120) (STJ25530)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SIN3A antibody (STJA0007718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Sin3B Polyclonal Antibody

Anti-SIN3B antibody (221-270 aa) (STJ94263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of Hela cell. 1, SIRT1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3B Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-SIRT1 antibody (13-62 aa) (STJ95667)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of NIH-3T3 cells using SIRT1 Polyclonal Antibody (STJ25533) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SIRT1 antibody (229-246) (STJ25533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human colon using SIRT1 Rabbit polyclonal antibody (STJ113751) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SIRT1 antibody (448-747) (STJ113751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SIRT1 antibody (448-747) (STJ119437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SIRT1 antibody (STJA0009872)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£49.00 - £261.00

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