• Western blot analysis of extracts of various cell lines, using SF3B1 antibody (STJ11103676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Confocal imaging of C2C12 cells using SF3B1 Rabbit monoclonal antibody (STJ11103676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded rat ovary using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens).
  • Confocal imaging of C6 cells using SF3B1 Rabbit monoclonal antibody (STJ11103676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded human appendix using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens).
  • Confocal imaging of HeLa cells using SF3B1 Rabbit monoclonal antibody (STJ11103676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry of paraffin-embedded mouse testis using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded human cervix cancer tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of C6 cells using SF3B1 rabbit monoclonal antibody (STJ11103676) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded mouse testis tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded mouse intestin tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded human colon tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded human colon carcinoma tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of SF3B1 in paraffin-embedded rat brain tissue using SF3B1 Rabbit monoclonal antibody (STJ11103676) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using SF3B1 Rabbit monoclonal antibody (STJ11103676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SF3B1 antibody (1-100) [S6MR] (STJ11103676)

SKU:
STJ11103676

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SF3B1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF3B1
Gene ID: 23451
Uniprot ID: SF3B1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SF3B1 (O75533).
Immunogen Sequence: MAKIAKTHEDIEAQIREIQG KKAALDEAQGVGLDSTGYYD QEIYGGSDSRFAGYVTSIAA TELEDDDDDYSSSTSLLGQK KPGYHAPVALLNDIPQSTEQ
Post Translational Modifications Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis. Citrullinated by PADI4.
Function Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92. May also be involved in the assembly of the 'E' complex. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable).
Protein Name Splicing Factor 3b Subunit 1
Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit
Sf3b155
Spliceosome-Associated Protein 155
Sap 155
Database Links Reactome: R-HSA-5250924
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Nucleus Speckle
During Mitosis
Transiently Dispersed From The Nuclear Speckles To The Cytoplasm
Alternative Antibody Names Anti-Splicing Factor 3b Subunit 1 antibody
Anti-Pre-Mrna-Splicing Factor Sf3b 155 Kda Subunit antibody
Anti-Sf3b155 antibody
Anti-Spliceosome-Associated Protein 155 antibody
Anti-Sap 155 antibody
Anti-SF3B1 antibody
Anti-SAP155 antibody

Information sourced from Uniprot.org

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