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Anti-SERPINE1/PAI-1 nanobody [SAA0793] (STJN000007)
General Information
| Short Description | Alpaca monoclonal nanobody anti-Plasminogen Activator Inhibitor 1 is suitable for use in Universaling and ELISA research applications. |
| Applications | Blocking/ELISA |
| Host | Alpaca |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SAA0793 |
| Isotype | VHH-8His-Cys-tag |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Purified by Nickel column. |
| Formulation | 0.01M PBS, pH 7.4. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at-20°C 12 months. Store at-80°C long term. |
Target Information
| Gene Symbol | SERPINE1 |
| Gene ID | 5054 |
| Uniprot ID | PAI1_HUMAN |
Additional Info
| Post Translational Modifications | Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond. |
| Function | Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis. |
| Protein Name | Plasminogen Activator Inhibitor 1PaiPai-1Endothelial Plasminogen Activator InhibitorSerpin E1 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-1368108Reactome: R-HSA-2173796Reactome: R-HSA-3000178Reactome: R-HSA-75205Reactome: R-HSA-9926550 |
| Cellular Localisation | Secreted |
| Alternative Nanobody Names | Anti-Plasminogen Activator Inhibitor 1 nanobodyAnti-Pai nanobodyAnti-Pai-1 nanobodyAnti-Endothelial Plasminogen Activator Inhibitor nanobodyAnti-Serpin E1 nanobodyAnti-SERPINE1 nanobodyAnti-PAI1 nanobodyAnti-PLANH1 nanobody |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Anti-SERPINE1/PAI-1 nanobody [SAA0793] (STJN000007)
This nanobody is directed against human SERPINE1/PAI-1 (UniProt P05121) and has confirmed reactivity with the human form of the protein. No cross-reactivity data for other species is provided.
This nanobody has been validated for blocking assays and ELISA. No other applications are listed in the current product data.
This nanobody is formatted as a VHH domain with a C-terminal 8His-Cys tag and is supplied unconjugated. The His-tag is compatible with Nickel-based detection or capture approaches in downstream assays.
This nanobody was purified by Nickel affinity column chromatography and has a confirmed purity of greater than 95% as determined by SDS-PAGE.
This nanobody is supplied at 1 mg/mL in 0.01M PBS, pH 7.4. For short-term use of 1 to 2 weeks, store at 4°C; for storage up to 12 months, store at -20°C; for long-term storage, keep at -80°C in a manual defrost freezer and avoid repeated freeze-thaw cycles.
No dissociation constant or affinity constant data is currently provided in the product specifications for this nanobody.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.