Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using Beta-Actin Mouse mAb (STJ11103683) at 1:2000-1:640000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Beta-Actin antibody [S3MR] (STJ11103683)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£135.50 - £249.50

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Anti-Beta-Actin antibody [SRM15105] (STJ11109835)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemistry analysis of Beta-Catenin in paraffin-embedded human skin using Beta-Catenin Mouse monoclonal antibody (STJ11103229) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-Beta-Catenin antibody (300-400 aa) [S9MM] (STJ11103229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Beta-Catenin antibody [14] (STJ16100314)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£989.50 - £1,291.50

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Anti-Beta-Catenin antibody [17C2] (STJ16101680)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,228.50

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Western blot analysis of Rat lung, using Osteocalcin (BGLAP) rabbit polyclonal antibody (STJ116843) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BGLAP antibody (1-100) (STJ116843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of U-87MG cells, using Osteocalcin (BGLAP) antibody (STJ27961) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BGLAP antibody (1-100) (STJ27961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BGLAP antibody (31-80 aa) (STJ192217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry of rat tissue with Anti-BGLAP primary antibody (STJ502017) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-BGLAP antibody (50-100) (STJ502017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Pig/Rat

£427.50

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Anti-BGLAP antibody (50-100) {Biotin} (STJ502018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Pig/Rat

£528.50

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Anti-BGLAP antibody (50-100) {FITC} (STJ502019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Pig/Rat

£528.50

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Immunofluorescence analysis of NIH-3T3 cells using Bid Rabbit polyclonal antibody (STJ22797) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Bid antibody (1-195) (STJ22797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and Bid Rabbit monoclonal antibody knockdown (KD) 293T (KD) cells, using Bid Rabbit monoclonal antibody (STJ11105057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-BID antibody (1-195) [S5057RM] (STJ11105057)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various cells using BID Polyclonal Antibody diluted at 1：1000

Anti-BID antibody (44-93 aa) (STJ91853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using BID monoclonal antibody against HeLa (1) , A431 (2) , Jurkat (3) , A549 (4) , HepG2 (5) , and HEK293 (6) cell lysate.

Anti-BID antibody [3C5] (STJ97864)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-BID antibody [PT0386R] (STJA0039148)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human

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Western blot analysis of extracts of various cell lines, using Survivin antibody (STJ11101752) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-BIRC5 antibody (1-100) [S2MR] (STJ11101752)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using Survivin Rabbit monoclonal antibody (STJ11102164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.

Anti-BIRC5 antibody (1-140) [S4MR] (STJ11102164)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of MCF7 cells using Survivin Rabbit polyclonal antibody (STJ11105318) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BIRC5 antibody (1-142) (STJ11105318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse fetal liver using Survivin Rabbit polyclonal antibody (STJ26172) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-BIRC5 antibody (1-142) (STJ26172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Survivin antibody (STJ11104675) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-BIRC5 antibody (1-142) [S4675RM] (STJ11104675)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Survivin Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-BIRC5 antibody (11-60 aa) (STJ95837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Survivin Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-BIRC5 antibody (86-135 aa) (STJ95838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-BIRC5 antibody (C-Term) (STJA0005747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Anti-BIRC5 antibody (STJ503164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, Survivin monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-BIRC5 antibody [Mix] (STJ97436)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-BIRC5 antibody {Biotin} (STJ503165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-BIRC5 antibody {FITC} (STJ503166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-BIRC5 nanobody [SAA1237] (STJN000043)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/SPR

Reactivity :

Human

£378.50 - £1,632.50

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Anti-BLK antibody (450-500) (STJ500258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-BLK antibody (450-500) {Biotin} (STJ500259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-BLK antibody (450-500) {FITC} (STJ500260)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various cells using Blk Polyclonal Antibody diluted at 1：500

Anti-BLK antibody (456-505 aa) (STJ91857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Blk monoclonal antibody against HEK293 (1) and BLK-hIgGFc transfected HEK293 (2) cell lysate.

Anti-BLK antibody [1E6] (STJ97869)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of A549 cells, using Bloom Syndrome Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-BLM antibody (65-114 aa) (STJ91858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-BLM nanobody [SAA0979] (STJN000235)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Anti-BLM nanobody [SAA1186] (STJN000234)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/SPR

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of K562 cells using BMAL1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-BMAL1 antibody (501-550 aa) (STJ96788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using BMAL1 monoclonal antibody against ARBTL-hIgGFc transfected HEK293 cell lysate.

Anti-BMAL1 antibody [1C5] (STJ97873)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-BMAL1 antibody [PT0696R] (STJA0039264)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of HeLa cells using Bmi1 antibody (STJ115433). Blue: DAPI for nuclear staining.

Anti-BMI1 antibody (120-326) (STJ115433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of K-562 cells, using 3 Mu g Bmi1 antibody (STJ22809). Western blot was performed from the immunoprecipitate using Bmi1 antibody (STJ22809) at a dilution of 1:1000.

Anti-BMI1 antibody (200-300) (STJ22809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Bmi1 antibody (STJ11101458) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-BMI1 antibody (227-326) [S8MR] (STJ11101458)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Anti-BMI1 antibody (STJ197427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis using Bmi-1 monoclonal antibody against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.

Anti-BMI1 antibody [3E3] (STJ97874)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-BMP2 antibody (1-100 aa) [SRM5252] (STJ11107154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using BMP2 Antibody (STJ114653) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-BMP2 antibody (1-100) (STJ114653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using BMP-2 Polyclonal Antibody diluted at 1：500

Anti-BMP2 antibody (226-275 aa) (STJ91863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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