• Western blot analysis of extracts of various cell lines, using AURKA antibody (STJ118174) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AURKA antibody (1-130) (STJ118174)

SKU:
STJ118174

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AURKA (1-130) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AURKA
Gene ID: 6790
Uniprot ID: AURKA_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human AURKA (NP_940837.1).
Immunogen Sequence: MDRSKENCISGPVKATAPVG GPKRVLVTQQFPCQNPLPVN SGQAQRVLCPSNSSQRIPLQ AQKLVSSHKPVQNQKQKQLQ ATSVPHPVSRPLNNTQKSKQ PLPSAPENNPEEELASKQKN EESKKRQWAL
Tissue Specificity Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Post Translational Modifications Activated by phosphorylation at Thr-288.this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis. Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome. Ubiquitinated by CHFR, leading to its degradation by the proteasome. Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. Ubiquitinated by the CUL3-KLHL18 ligase leading to its activation at the centrosome which is required for initiating mitotic entry. Ubiquitination mediated by CUL3-KLHL18 ligase does not lead to its degradation by the proteasome.
Function Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Important for microtubule formation and/or stabilization. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Inhibits cilia outgrowth. Required for cilia disassembly via phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin. Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1.
Protein Name Aurora Kinase A
Aurora 2
Aurora/Ipl1-Related Kinase 1
Ark-1
Aurora-Related Kinase 1
Breast Tumor-Amplified Kinase
Ipl1- And Aurora-Related Kinase 1
Serine/Threonine-Protein Kinase 15
Serine/Threonine-Protein Kinase 6
Serine/Threonine-Protein Kinase Ayk1
Serine/Threonine-Protein Kinase Aurora-A
Database Links Reactome: R-HSA-174178
Reactome: R-HSA-2565942
Reactome: R-HSA-4615885
Reactome: R-HSA-6804114
Reactome: R-HSA-6804756
Reactome: R-HSA-8854050
Reactome: R-HSA-8854518
Reactome: R-HSA-8854521
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole
Centriole
Cell Projection
Neuron Projection
Cilium
Cilium Basal Body
Basolateral Cell Membrane
Detected At The Neurite Hillock In Developing Neurons
Localizes At The Centrosome In Mitotic Cells From Early Prophase Until Telophase
But Also Localizes To The Spindle Pole Mts From Prophase To Anaphase
Colocalized With Sirt2 At Centrosome
Moves To The Midbody During Both Telophase And Cytokinesis
Associates With Both The Pericentriolar Material (Pcm) And Centrioles
The Localization To The Spindle Poles Is Regulated By Aaas
Alternative Antibody Names Anti-Aurora Kinase A antibody
Anti-Aurora 2 antibody
Anti-Aurora/Ipl1-Related Kinase 1 antibody
Anti-Ark-1 antibody
Anti-Aurora-Related Kinase 1 antibody
Anti-Breast Tumor-Amplified Kinase antibody
Anti-Ipl1- And Aurora-Related Kinase 1 antibody
Anti-Serine/Threonine-Protein Kinase 15 antibody
Anti-Serine/Threonine-Protein Kinase 6 antibody
Anti-Serine/Threonine-Protein Kinase Ayk1 antibody
Anti-Serine/Threonine-Protein Kinase Aurora-A antibody
Anti-AURKA antibody
Anti-AIK antibody
Anti-AIRK1 antibody
Anti-ARK1 antibody
Anti-AURA antibody
Anti-AYK1 antibody
Anti-BTAK antibody
Anti-IAK1 antibody
Anti-STK15 antibody
Anti-STK6 antibody

Information sourced from Uniprot.org

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