Gene Expression And Transcription | Research Area | St John's Laboratory

Immunofluorescence analysis of U2OS cells using TGF beta 1 Rabbit polyclonal antibody (STJ29875) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TGFB1 antibody (291-390) (STJ29875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using TGF beta 1 Rabbit polyclonal antibody (STJ117297) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TGFB1 antibody (30-390) (STJ117297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates of K562 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-TGF β1 (PT2173) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 44kDaObserved band size: 44kDa

Anti-TGFB1 antibody (300-390) [PT2173] (STJA0006239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-TGFB1 antibody (336-385 aa) (STJ95997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TGFB1 antibody [1F12] (STJ196644)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of K-562, using TGFbeta1 antibody (STJ11104240) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TGFB1 antibody [S4240RM] (STJ11104240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using TGIF1 Rabbit polyclonal antibody (STJ11102821) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TGIF1 antibody (250-340) (STJ11102821)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TGIF1 antibody (C-Term) (STJ96000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TGIF2 antibody (STJ190900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using THBS1 antibody (STJ119258) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-THBS1 antibody (150-250) (STJ119258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of THBS1 in paraffin-embedded rat spleen using THBS1 Rabbit polyclonal antibody (STJ25844) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-THBS1 antibody (19-352) (STJ25844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Thrombospondin 1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-THBS1 antibody (547-1170 aa) [R06-5F3] (STJA0012618)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

£264.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-THBS1 antibody (STJ196535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Nuclear Matrix Protein p84 (THOC1) (THOC1) Rabbit mAb (STJ11102469) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-THOC1 antibody (350-450) [S9MR] (STJ11102469)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunocytochemistry analysis of Nuclear Matrix Protein p84 (green) in Hela using Nuclear Matrix Protein p84 antibody, and DAPI (blue)

Anti-THOC1 antibody (38-49 aa) [R05-1A8] (STJA0011704)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£246.50

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Western blot analysis of various lysates using Nuclear Matrix Protein p84 (THOC1) Rabbit pAb (STJ110478) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-THOC1 antibody (428-657) (STJ110478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-THOC1 antibody (STJA0007087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using THOC2 Rabbit polyclonal antibody (STJ11100288) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-THOC2 antibody (1-270) (STJ11100288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-THOC2 antibody (STJA0007626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using THOC3 Rabbit polyclonal antibody (STJ115196) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-THOC3 antibody (1-200) (STJ115196)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using THOC5 Rabbit polyclonal antibody (STJ113240) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-THOC5 antibody (150-370) (STJ113240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using THOC5 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-THOC5 antibody (561-610 aa) (STJ93097)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using THOC6 Rabbit polyclonal antibody (STJ119802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-THOC6 antibody (50-150) (STJ119802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-THOC6 antibody (79-129) (STJA0006610)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using THOC7 Rabbit polyclonal antibody (STJ115655) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-THOC7 antibody (1-204) (STJ115655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-THOC7 antibody (STJA0007918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Thyroid Hormone Receptor alpha Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-THRA antibody (10-59 aa) (STJ96120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-THRA antibody (119-199 aa) [SRM] (STJ11107153)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded mouse lung using THRA Rabbit polyclonal antibody (STJ27561) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-THRA antibody (138-489) (STJ27561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Mouse pancreas, using TR Beta 1/THRB Rabbit polyclonal antibody (STJ25847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-THRB antibody (1-263) (STJ25847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using TR Beta 1/THRB antibody (STJ11104880) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-THRB antibody (1-91) [S4880RM] (STJ11104880)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Thyroid Hormone Receptor beta Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-THRB antibody (11-60 aa) (STJ96121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~50 kDa TR-Alpha 1 and the ~58 kDa TR-Alpha 2 protein.

Anti-Thyroid Hormone Receptor Alpha 1/2 antibody (N-Term) [1718] (STJA0003801)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Rat/Canine/Mouse/Non-Human Primates

£454.50

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Anti-Thyroid Hormone Receptor Alpha 1/2 antibody (N-Term) [2103] (STJA0003800)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Rat/Canine/Mouse/Non-Human Primates

£454.50

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~58 kDa TR-Alpha 2 protein.

Anti-Thyroid Hormone Receptor Alpha 2 antibody (N-Term) [1330] (STJA0003802)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Rat/Bovine/Mouse/Non-Human Primates

£454.50

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Western blot of hippocampal lysate showing specific immunolabeling of the ~55 kDa TR-Beta protein.

Anti-Thyroid Hormone Receptor Beta antibody (N-Term) [2386] (STJA0003803)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£454.50

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Immunofluorescence analysis of U2OS cells using TIGAR Rabbit polyclonal antibody (STJ11103300) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-TIGAR antibody (100-200) (STJ11103300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-TIGAR antibody (STJ193103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using [KD Validated] ZO-1 Rabbit polyclonal antibody (STJ11105590) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-TJP1 antibody (1500-1650) (STJ11105590)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-ZO1 antibody at 1:2500 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-TJP1 antibody (1580aa C-Term) (STJ140055)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Human placental mesenchymal stromal cells modulate IFN-γ and IL-10 secretion by CD4+T cells via CD73, and alleviate intestinal damage in mice with graft-versus-host disease. INTERNATIONAL IMMUNOPHARMACOLOGY Xiying Luan WB Human, mouse intestine, colon NCM460 cell, Caco-2 cell

Anti-TJP1 antibody (1600-1700 aa) (STJ191514)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Pig

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using ZO-1 antibody (STJ113790) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TJP1 antibody (18-1748) (STJ113790)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of MCF7 cells using ZO-1 Rabbit polyclonal antibody (STJ25857) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TJP1 antibody (18-1748) (STJ25857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-TJP1 antibody (18-299 aa) (STJ11106811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-TJP1 antibody (STJ11106849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Immunofluorescence analysis of rat spleen using TNFRSF10A Polyclonal Antibody (STJ114414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TNFRSF10A antibody (269-468) (STJ114414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of DU145 cells, using TNFRSF10A antibody (STJ28189) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TNFRSF10A antibody (269-468) (STJ28189)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using DR4 Polyclonal Antibody

Anti-TNFRSF10A antibody (401-450 aa) (STJ92775)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

Choose Options