Disease | Research Area | St John's Laboratory

Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0. 01M containing Triton X 0. 005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to extracellular domain of human CD36 (Fatty acid translocase) : IgG (STJ13100092) diluted 1: 100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1: 100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Anti-CD36 antibody (ECD) (STJ13100092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-CD36 antibody (ECD) (STJ13100102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Marmoset/Rat/Mouse

£331.50

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Anti-CD36 antibody (ECD) (STJ13100903)

Clonality :

Polyclonal

Host :

Goat

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-CD36 antibody (STJ500463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human

£427.50

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Anti-CD36 antibody [185-1G2] (STJ16102561)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Human ErythroLeukemia cell line HEL were stained with Anti-CD36 antibody (STJA0000953) (filled gray histogram). Unstained Human ErythroLeukemia cell line HEL (empty black histogram) were used as control.

Anti-CD36 antibody [5-271] {APC} (STJA0000953)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£142.50 - £331.50

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Anti-CD36 antibody [5-271] {EF488} (STJA0016436)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £352.50

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Anti-CD36 antibody [5-271] {EF700} (STJA0016437)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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Anti-CD36 antibody [5-271] {FITC} (STJA0001928)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£131.50 - £279.50

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Anti-CD36 antibody [5-271] {PE} (STJA0001929)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£142.50 - £310.50

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Anti-CD36 antibody [EBS-CD-023] (STJ16101137)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Anti-CD36 antibody [EBS-CD-024] (STJ16102587)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Anti-CD36 antibody [FA6-152] (STJ16100295)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/ELISA/IF/IP

Reactivity :

Human

£595.50

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Anti-CD36 antibody {Biotin} (STJ500464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human

£528.50

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Anti-CD36 antibody {FITC} (STJ500465)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human

£528.50

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Western blot analysis of extracts of various cell lines, using CD36/SR-B3 antibody (STJ11102904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CD36/SR-B3 antibody (93-165) (STJ11102904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-CD39 antibody [A1] {EF780} (STJA0016438)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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Anti-CD39/ENTPD1 antibody [A1] {AF488} (STJA0001934)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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Anti-CD39/ENTPD1 antibody [A1] {AF647} (STJA0001935)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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Human peripheral blood lymphocytes were stained with Anti-CD39 antibody (STJA0000955) (filled gray histogram) or Mouse IgG1 Isotype Control APC (empty black histogram).

Anti-CD39/ENTPD1 antibody [A1] {APC} (STJA0000955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£142.50 - £352.50

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Human peripheral blood lymphocytes were stained with Anti-CD39 antibody (STJA0000954) (filled gray histogram). Unstained lymphocytes (empty black histogram) were used as control.

Anti-CD39/ENTPD1 antibody [A1] {FITC} (STJA0000954)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£131.50 - £279.50

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Anti-CD39/ENTPD1 antibody [A1] {PE/Cyanine5.5} (STJA0001932)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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Anti-CD39/ENTPD1 antibody [A1] {PE/Cyanine5} (STJA0001931)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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Human peripheral blood lymphocytes were stained with Anti-CD39 antibody (STJA0000956) (filled gray histogram) or Mouse IgG1 Isotype Control PE/Cy7 (empty black histogram).

Anti-CD39/ENTPD1 antibody [A1] {PE/Cyanine7} (STJA0000956)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£159.50 - £377.50

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Anti-CD39/ENTPD1 antibody [A1] {PE} (STJA0001930)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£131.50 - £310.50

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Anti-CD3G antibody (131-181) (STJ500451)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£427.50

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Anti-CD3G antibody (131-181) {Biotin} (STJ500466)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Anti-CD3G antibody (131-181) {FITC} (STJ500467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse

£528.50

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Western blot analysis of various lysates, using CD3G antibody (STJ11103929) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-CD3G antibody (23-116) (STJ11103929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Flow cytometry: 1X10^6 Raji cells (negative control, left) and Jurkat cells (right) were intracellularly-stained with CD3G Rabbit monoclonal antibody (STJ11103551, 2. 5 Mu g/mL orange line) or ABflo® 488 Rabbit IgG isotype control ( 2. 5 Mu g/mL, blue line). Non-fluorescently stained cells were used as blank control (red line).

Anti-CD3G antibody (83-182) [S1MR] (STJ11103551)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/FC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD3G antibody (STJ192944)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD4 (PTR2055) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: JurkatPredicted band size: 51kDaObserved band size: 51kDa

Anti-CD4 antibody (1-100) [PTR2055] (STJA0006231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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Human tonsil stained with anti-CD4 using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of T cells.

Anti-CD4 antibody (216-396aa) [ZM180] (STJ180495)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£181.50 - £544.50

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Immunofluorescence analysis of Jurkat cells using CD4 Rabbit polyclonal antibody (STJ114878) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CD4 antibody (26-200) (STJ114878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded mouse spleen using CD4 Rabbit polyclonal antibody (STJ114879) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD4 antibody (26-200) (STJ114879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Jurkat cells using CD4 Rabbit polyclonal antibody (STJ26171) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD4 antibody (26-317) (STJ26171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot with Anti-CD4 Antibody (STJ500468) with peptide on at 1:500 dilution in buffer.

Anti-CD4 antibody (270-320) (STJ500468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse

£427.50

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Anti-CD4 antibody (270-320) {Biotin} (STJ500470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse

£528.50

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Anti-CD4 antibody (270-320) {FITC} (STJ500471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Mouse

£528.50

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Western blot analysis of various cells using CD4 Polyclonal Antibody diluted at 1：500

Anti-CD4 antibody (401-450 aa) (STJ92127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry of mouse spleen with Anti-CD4 primary antibody (STJ500469) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD4 antibody (437-458) (STJ500469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-CD4 antibody (437-458) {Biotin} (STJ500472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-CD4 antibody (437-458) {FITC} (STJ500473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Endogenous CD4 detected at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-CD4 antibody (50-235aa ECD) (STJ140023)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, CD4 monoclonal antibody (11A1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CD4 antibody [11A1] (STJ96972)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CD4 antibody [4B12] (STJ16101710)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,130.50

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Flow cytometric analysis of blood T cells using CD4 monoclonal antibody (M2) and negative control (M1).

Anti-CD4 antibody [B486A1] (STJ97926)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-CD4 antibody [EDU-2] (STJ16101105)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human/Chimpanzee/Orangutan/Macaque

£287.50 - £1,324.50

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