• Immunohistochemistry analysis of paraffin-embedded human prostate using BTD antibody (STJ28206) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using BTD antibody (STJ28206) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using BTD antibody (STJ28206) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using BTD antibody (STJ28206) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-BTD antibody (284-543) (STJ28206)

SKU:
STJ28206

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BTD (284-543) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BTD
Gene ID: 686
Uniprot ID: BTD_HUMAN
Immunogen Region: 284-543
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 284-543 of human BTD (NP_000051.1).
Immunogen Sequence: IQKAFAVAFGINVLAANVHH PVLGMTGSGIHTPLESFWYH DMENPKSHLIIAQVAKNPVG LIGAENATGETDPSHSKFLK ILSGDPYCEKDAQEVHCDEA TKWNVNAPPTFHSEMMYDNF TLVPVWGKEGYLHVCSNGLC CYLLYERPTLSKELYALGVF DGLHTVHGTYYIQVCALVRC GGLGFDTCGQEITEATGIFE FHLWGNFSTSYIFPLFLTSG MTLEVPDQLGWENDHYFLR
Function Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.
Protein Name Biotinidase
Biotinase
Database Links Reactome: R-HSA-196780
Reactome: R-HSA-3371598
Cellular Localisation Secreted
Extracellular Space
Alternative Antibody Names Anti-Biotinidase antibody
Anti-Biotinase antibody
Anti-BTD antibody

Information sourced from Uniprot.org

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