• Immunofluorescence analysis of H9C2 cells using BRMS1 Rabbit polyclonal antibody (STJ117065) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of BRMS1 in paraffin-embedded mouse liver using BRMS1 Rabbit polyclonal antibody (STJ117065) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BRMS1 in paraffin-embedded human colon using BRMS1 Rabbit polyclonal antibody (STJ117065) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of BRMS1 in paraffin-embedded rat liver using BRMS1 Rabbit polyclonal antibody (STJ117065) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using BRMS1 Rabbit polyclonal antibody (STJ117065) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BRMS1 antibody (90-210) (STJ117065)

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STJ117065

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-BRMS1 (90-210) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRMS1
Gene ID: 25855
Uniprot ID: BRMS1_HUMAN
Immunogen Region: 90-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human BRMS1 (NP_056214.1).
Immunogen Sequence: LEEVGAERAPEYTEPLGGLQ RSLKIRIQVAGIYKGFCLDV IRNKYECELQGAKQHLESEK LLLYDTLQGELQERIQRLEE DRQSLDLSSEWWDDKLHARG SSRSWDSLPPSKRKKAPLVS G
Tissue Specificity Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
Post Translational Modifications Ubiquitinated by a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing SPOP, leading to proteasomal degradation.
Function Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
Protein Name Breast Cancer Metastasis-Suppressor 1
Database Links Reactome: R-HSA-3214815
Reactome: R-HSA-9679191
Cellular Localisation Nucleus
Cytoplasm
Predominantly Nuclear
Alternative Antibody Names Anti-Breast Cancer Metastasis-Suppressor 1 antibody
Anti-BRMS1 antibody

Information sourced from Uniprot.org

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