Developmental Biology | Research Area | St John's Laboratory

Western blot analysis using OTX2 monoclonal antibody against HepG2 (1) , Jurkat (2) , and NTERA-2 (3) cell lysate.

Anti-OTX2 antibody [1H12C4B5] (STJ98292)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-p21 antibody [DCS-60.2] (STJ16100407)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£517.50 - £1,055.50

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Immunohistochemistry analysis of paraffin embedded human cervical cancer using p21 at dilution of 1:1000

Anti-P21 antibody [SM4972] (STJA0004972)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Immunohistochemistry analysis of p21. Sample:Human cervical carcinoma, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2 min. Primary antibody: 1:600, 4C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG, Ready to use, RT, 20min.

Anti-p21 antibody [SR0522] (STJA0010522)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-P21 antibody [WA-1] (STJA0018408)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC

Reactivity :

Human

£1,253.50

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Anti-p21/WAF1 antibody [WA-1] (STJ16100762)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/IF/IHC

Reactivity :

Human/Chimpanzee/Monkey/Mouse/Rat

£287.50 - £1,324.50

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Human colon adenocarcinoma stained with anti-p21 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells.

Anti-p21WAF1 antibody [ZR288] (STJ180533)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£176.50 - £654.50

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Immunohistochemistry analysis of paraffin-embedded Human placenta using p38 MAPK Rabbit polyclonal antibody (STJ11102677) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-p38 MAPK antibody (261-360) (STJ11102677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-P53 antibody [BP53+12] (STJ16100968)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/IHC-P/IF

Reactivity :

Human

£521.50

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Anti-p53 antibody [BP53-12] (STJ16100743)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Chicken/Dog/Hamster/Human/Monkey

£287.50 - £1,324.50

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Anti-p53 antibody [BP53.12] (STJA0018375)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£1,230.50

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Anti-p53 antibody [BP53.12] {FITC} (STJA0018374)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£1,981.50

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Anti-p53 antibody [DO7] (STJ16100410)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,009.50 - £1,055.50

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Anti-p53 antibody [SRM] (STJ11107142)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

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Human colon carcinoma infiltrating fat stained with anti-p53 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of carcinoma cells.

Anti-p53 antibody [ZR153] (STJ180535)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £511.50

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Anti-PABPC1 antibody (212-262 aa) (STJ192910)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates, using polyclonal antibodyPC1 antibody (STJ117072) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PABPC1 antibody (350-450) (STJ117072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PABPN1 antibody (STJ27837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PABPN1 antibody (1-100) (STJ27837)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PABPN1 rabbit monoclonal antibody (STJ11101447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PABPN1 antibody (1-100) [S7MR] (STJ11101447)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-PABPN1 antibody (16-28 aa) [R02-8B1] (STJA0011740)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

£246.50

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PABPN1 antibody (170-250 aa) (STJ191174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-PAG/Cbp antibody [MEM-255] (STJ16100482)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IHC-P

Reactivity :

Human

£537.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PAGR1 antibody (163-213 aa) (STJ194476)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PAIP1 antibody (STJ27838) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PAIP1 antibody (1-260) (STJ27838)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-PAIP1 antibody (100-180 aa) (STJ191176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of NIH-3T3 cells using PAK1 Rabbit monoclonal antibody (STJ11101728). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PAK1 antibody (1-100) [S8MR] (STJ11101728)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using PAK1 antibody (STJ114900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PAK1 antibody (130-250) (STJ114900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of various cells using PAK Alpha Polyclonal Antibody diluted at 1：500

Anti-PAK1 antibody (178-227 aa) (STJ94941)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using PAK1 Antibody (STJ24888) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PAK1 antibody (200-300) (STJ24888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from wild type (WT) and PAK2 knockout (KO) HeLa cells, using [KO Validated] PAK2 Rabbit polyclonal antibody (STJ11100066) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PAK2 antibody (1-380) (STJ11100066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using PAK2 antibody (STJ29472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PAK2 antibody (120-240) (STJ29472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 3T3 cells using PAK Gamma Polyclonal Antibody

Anti-PAK2 antibody (5-54 aa) (STJ94946)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using PAK Gamma monoclonal antibody against HeLa (1) , Jurkat (2) , A549 (3) , HEK293 (4) and K562 (5) cell lysate.

Anti-PAK2 antibody [3B5] (STJ98304)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of NIH-3T3 cells using PAK3 Rabbit polyclonal antibody (STJ11101194) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PAK3 antibody (1-100) (STJ11101194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using PAK3 Rabbit monoclonal antibody (STJ11101854) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PAK3 antibody (1-100) [S4MR] (STJ11101854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-PAK3 antibody (121-170 aa) (STJ94945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from wild type (WT) and PAK4 knockout (KO) 293T cells, using [KO Validated] PAK4 Rabbit pAb (STJ11100032) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-PAK4 antibody (342-591) (STJ11100032)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of extracts of LNCaP cells, using PAK4 antibody (STJ113249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PAK4 antibody (450-550) (STJ113249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from U-87MG cells, using PAK5 Rabbit polyclonal antibody (STJ118317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PAK5 antibody (600-700) (STJ118317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using PAK5 Polyclonal Antibody

Anti-PAK5 antibody (661-710 aa) (STJ94938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using PAK6 Rabbit polyclonal antibody (STJ110131) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PAK6 antibody (1-270) (STJ110131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PAK6 antibody (100-180 aa) (STJ190138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-PAK6 antibody (N-Term) (STJA0005510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£367.50

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Anti-Pan-Alpha Tubulin antibody [SM5020] (STJA0005020)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using Cav pan α1Rabbit pAb diluted at 1:200.

Anti-Pan-CACNA1C antibody (1288-1338 aa) (STJ197418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin embedded human pancreas using Pan-cadherin at dilution of 1:200

Anti-Pan-Cadherin antibody [SM4933] (STJA0004933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of Jurkat cells using Keratin-pan Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Pan-KRT2 antibody (151-200 aa) (STJ96694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human lung squamous cell carcinoma (A) , normal hepatocyte (B) , colon adenocacinoma, normal stomach tissue (D) , showing cytoplasmic and membrane localization with DAB staining using Cytokeratin (Pan) Monoc

Anti-Pan-KRT5 antibody [7H8] (STJ97994)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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