Anti-FOXA2 antibody [R07-3H4] (STJA0036321)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human FOXA2
STJA0036321
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General Information

Short DescriptionRabbit monoclonal anti-FOXA2 for use in WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ICC/IF
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR07-3H4
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Chromatography
Dilution RangeWB 1:500-1:1000
IF 1:50-1:200
FormulationRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolFOXA2
Gene ID3170
Uniprot IDFOXA2_HUMAN
ImmunogenA synthesized peptide derived from human FOXA2

Additional Info

Post Translational Modifications Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
Function Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-ACAATTAGTTGTAGCTTCT-3'. In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs.FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Protein Name Hepatocyte Nuclear Factor 3-Beta
Hnf-3-Beta
Hnf-3b
Forkhead Box Protein A2
Transcription Factor 3b
Tcf-3b
Database Links Reactome: R-HSA-210745
Reactome: R-HSA-9796292
Reactome: R-HSA-9823730
Reactome: R-HSA-9925561
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between The Nucleus And Cytoplasm In A Crm1-Dependent Manner
In Response To Insulin Signaling Via Akt1 Is Exported From The Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 3-Beta antibody
Anti-Hnf-3-Beta antibody
Anti-Hnf-3b antibody
Anti-Forkhead Box Protein A2 antibody
Anti-Transcription Factor 3b antibody
Anti-Tcf-3b antibody
Anti-FOXA2 antibody
Anti-HNF3B antibody
Anti-TCF3B antibody

Information sourced from Uniprot.org

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