Developmental Biology | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, CD41 monoclonal antibody (Q90) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-ITGA2B antibody [Q90] (STJ97008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Western blot analysis of extracts from wild type (WT) and Integrin alpha 5 (ITGA5/CD49e) knockdown (KD) HeLa cells, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11104927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ITGA5 antibody (42-995 aa) [S4927RM] (STJ11104927)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ11104662) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ITGA5 antibody (42-995) (STJ11104662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of Hela cells using Integrin Alpha 5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ITGA5 antibody (561-610 aa) (STJ97284)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Human placenta using Integrin alpha 5 (ITGA5/CD49e) antibody (STJ27965) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ITGA5 antibody (750-1000) (STJ27965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Integrin Alpha 5 monoclonal antibody against HeLa, HUVEC cell lysate.

Anti-ITGA5 antibody (N-Term) (STJ98510)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC

Reactivity :

Human/Mouse/Rat/Cow

£144.00 - £348.00

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Western blot analysis using Integrin Alpha 5 monoclonal antibody against ITGA5-hIgGFc transfected HEK293 cell lysate.

Anti-ITGA5 antibody [10F6] (STJ98181)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various lysates using ITGA8 Rabbit polyclonal antibody (STJ115023) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ITGA8 antibody (907-1000) (STJ115023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using ITGA9 antibody (STJ110006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ITGA9 antibody (30-250) (STJ110006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using ITGA9 antibody (STJ115942) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ITGA9 antibody (30-250) (STJ115942)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-ITGA9 antibody (40-120 aa) (STJ190340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunoprecipitation analysis of 200 Mu g extracts of C6 cells using 3 Mu g Integrin alpha V (ITGAV/CD51) antibody (STJ24252). Western blot was performed from the immunoprecipitate using Integrin alpha V (ITGAV/CD51) antibody (STJ24252) at a dilution of 1:2000.

Anti-ITGAV antibody (1021-1034) (STJ24252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of Integrin alpha V (green) in A549 using Integrin alpha V antibody, and DAPI (blue).

Anti-ITGAV antibody (32-236 aa) [R02-6G3] (STJA0010733)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

£264.50

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Western blot analysis of various cells using Integrin Alpha V Polyclonal Antibody diluted at 1：1000

Anti-ITGAV antibody (755-804 aa) (STJ93730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Integrin alpha V (ITGAV/CD51) (ITGAV/CD51) antibody (STJ11101608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ITGAV antibody (949-1048) [S8MR] (STJ11101608)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/FC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of NIH-3T3 cells using Integrin-Beta 1/CD29 Rabbit polyclonal antibody (STJ111129) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ITGB1 antibody (1-728) (STJ111129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Jurkat cells, using Integrin beta1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ITGB1 antibody (746-795 aa) (STJ93732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ITGB1 antibody (Extracellular region) [M004] (STJA0005446)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£390.50

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Anti-ITGB1 antibody (Extracellular region) [M006] (STJA0005447)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£390.50

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Anti-ITGB1 antibody (Extracellular region) [M032] (STJA0005448)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

£365.50

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Anti-ITGB1 antibody (Extracellular region) [M041] (STJA0005449)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human

£390.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD61 (ABT032) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HUVECPredicted band size: 70kDaObserved band size: 70kDa

Anti-ITGB3 antibody (1-100 aa) [PT0052] (STJ196789)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunohistochemistry of baboon kidney with Anti-ITGB3 primary antibody (STJ501482) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ITGB3 antibody (1-50) (STJ501482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£427.50

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Anti-ITGB3 antibody (1-50) {Biotin} (STJ501483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-ITGB3 antibody (1-50) {FITC} (STJ501484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Western blot analysis of various lysates using ITGB3 Rabbit polyclonal antibody (STJ11105487) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB3 antibody (21-718) (STJ11105487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) antibody (STJ114863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ITGB3 antibody (609-788) (STJ114863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Integrin beta 3 (ITGB3/CD61) antibody (STJ11101610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ITGB3 antibody (689-788) [S0MR] (STJ11101610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/FC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Whole cell lysates of A549 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Integrin β3 (PTR2555) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: A549Predicted band size: 87kDaObserved band size: 72kDa

Anti-ITGB3 antibody (700-788) [PTR2555] (STJA0006262)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Integrin Beta 3 Polyclonal Antibody diluted at 1：500

Anti-ITGB3 antibody (739-788 aa) (STJ93735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse bone marrow using Integrin beta 3 (ITGB3/CD61) Rabbit polyclonal antibody (STJ26210) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ITGB3 antibody (80-788) (STJ26210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ITGB3 antibody (Extracellular region) [M581] (STJA0005450)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£365.50

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Western blot detection of Integrin beta 3 (N-terminus) in 15ug and 25ug Leukocyte whole cell lysate using Integrin beta 3 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size:110KDa.Observed band size:110KDa.

Anti-ITGB3 antibody (N-Term) [3C12-G2-E4-H9] (STJ99085)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Anti-ITGB3 antibody [ABT134R] (STJ197069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of various lysates using ITSN1 polyclonal antibody (STJ11100331) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ITSN1 antibody (750-850) (STJ11100331)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-ITSN1 antibody (STJA0007896)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using IVL antibody (STJ110332) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-IVL antibody (416-585) (STJ110332)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using Involucrin Polyclonal Antibody

Anti-IVL antibody (536-585 aa) (STJ93742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using JAG1 antibody (STJ114606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-JAG1 antibody (34-180) (STJ114606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of extracts of Mouse brain, using JAG1 antibody (STJ114627) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-JAG1 antibody (34-180) (STJ114627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunofluorescence analysis of human-liver tissue. 1, Jagged1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-JAG1 antibody (981-1030 aa) (STJ96766)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using c-Jun Rabbit polyclonal antibody (STJ113779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Jun antibody (1-70) (STJ113779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of human-lung tissue. 1, AP-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-JUN antibody (58-107 aa) (STJ91616)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using c-Jun Rabbit polyclonal antibody (STJ24273) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-JUN antibody (90-230) (STJ24273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-JUN antibody (STJA0010216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-JUN antibody (STJA0010217)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of c-JUN in CHO-K1 transfected by PEGFP-C1-c-JUN (A) and CHO-K1 cell lysate (B) cell lysate using c-JUN mouse mAb (1:1000 diluted).Predicted band size:43/48KDa.Observed band size:43/48KDa.

Anti-JUN antibody [3B3-A5-D7-F7] (STJ99131)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis using AP-1 monoclonal antibody against NIH/3T3 (1) and Cos7 (2) cell lysate.

Anti-JUN antibody [5B1] (STJ97829)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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