Cell Cycle | Research Area | St John's Laboratory

Human urothelial carcinoma stained with anti-thymidylate synthase (TS) antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells.

Anti-Thymidylate Synthase antibody [ZR245] (STJ180544)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$218.03 - $776.93

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Western blot analysis of extracts of various cell lines, using TIN2/TIN2/TINF2 antibody (STJ28852) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TINF2 antibody (202-451) (STJ28852)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various lysates using TIN2/TINF2 Rabbit mAb (STJ11102539) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-TINF2 antibody (210-309) [S9MR] (STJ11102539)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of lysates from HUVEC cells, using TINF2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-TINF2 antibody (71-120 aa) (STJ96027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using Thymidine Kinase 1 Rabbit monoclonal antibody (STJ11101426) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TK1 antibody (1-100) [S6MR] (STJ11101426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-TK1 antibody (1-50 aa) (STJ96013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using Thymidine Kinase 1 antibody (STJ27579) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TK1 antibody (15-194) (STJ27579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from HeLa and COS7 cells, using KITH Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-TK1 antibody (161-210 aa) (STJ96012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Monkey

$64.80 - $340.20

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Western blot analysis of various lysates using TNPO1 Rabbit pAb (STJ111699) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-TNPO1 antibody (790-890) (STJ111699)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using TNPO1 Rabbit pAb (STJ115950) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 5s.

Anti-TNPO1 antibody (C-Term) (STJ115950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-TNPO1 antibody (STJ191554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using Topo II Alpha Polyclonal Antibody diluted at 1：1000. Secondary antibody was diluted at 1:20000

Anti-TOP2A antibody (1-50 aa) (STJ96066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using DNA topoisomerase II alpha (TOP2A) antibody (STJ118868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TOP2A antibody (1100-1200) (STJ118868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot with Anti-TOP2A Antibody (STJ503357) with TOPO2A peptide. Antibody at 1:500 dilution in buffer.

Anti-TOP2A antibody (1220-1270) (STJ503357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-TOP2A antibody (1220-1270) {Biotin} (STJ503358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-TOP2A antibody (1220-1270) {FITC} (STJ503359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Immunofluorescence analysis of U-2 OS cells using DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A) ) Rabbit polyclonal antibody (STJ11103810) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TOP2A antibody (1314-1503) (STJ11103810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Topoisomerase IIα (ABT272) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 174kDaObserved band size: 174kDa

Anti-TOP2A antibody (1400-1531 aa) [ABT272] (STJ197194)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

$234.90 - $340.20

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Immunohistochemistry analysis of Topo II Alpha in paraffin-embedded human cervix cancer tissue using Topo II Alpha Rabbit polyclonal antibody (STJ11105362) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M Tris-EDTA buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-TOP2A antibody (1431-1531) (STJ11105362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using DNA DNA topoisomerase II alpha (TOP2A) Rabbit monoclonal antibody (STJ11102067) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TOP2A antibody (1432-1531) [S7MR] (STJ11102067)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Anti-TOP3A antibody (1-100) (STJ11102823)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

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Western blot analysis of 293T using Topo III Alpha antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-TOP3A antibody (350-430 aa) (STJ97619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded rat lung using TOPBP1 antibody (STJ114448) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TOPBP1 antibody (1-100) (STJ114448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using TOPBP1 Rabbit pAb (STJ114440) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 50s.

Anti-TOPBP1 antibody (1253-1522) (STJ114440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of HCT116 cells, using TOPBP1 antibody (STJ28348) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TOPBP1 antibody (1253-1522) (STJ28348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-TOPBP1 antibody (STJA0010274)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with anti-Topoisomerase IIa antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

Anti-Topoisomerase II alpha antibody (1431-1531aa) [ZR94] (STJ180481)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$245.03 - $949.73

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Immunocytochemistry analysis of p53 (green) in MCF-7 using p53 antibody, and DAPI (blue)

Anti-TP53 antibody (1-101 aa) [R07-6A7] (STJA0011734)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

$332.78

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g p53 antibody (STJ11104447). Western blot was performed from the immunoprecipitate using p53 antibody (STJ11104447) at a dilution of 1:3000.

Anti-TP53 antibody (1-393) (STJ11104447)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human

$174.83 - $867.38

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 3 Mu g p53 antibody (STJ11104449). Western blot was performed from the immunoprecipitate using p53 antibody (STJ11104449) at a dilution of 1:2000.

Anti-TP53 antibody (1-393) (STJ11104449)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation of p53 in 200 µg extracts from 293T cells using 3 µg p53 Rabbit polyclonal antibody (STJ25926). Western blot analysis was performed using p53 Rabbit polyclonal antibody (STJ25926) at 1:3000 dilution.

Anti-TP53 antibody (1-393) (STJ25926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 200 Mu g extracts of U-251MG cells using 3 Mu g p53 Rabbit polyclonal antibody (STJ25927). Western blot was performed from the immunoprecipitate using p53 Rabbit polyclonal antibody (STJ25927) at a dilition of 1:1000.

Anti-TP53 antibody (1-393) (STJ25927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] p53 Rabbit monoclonal antibody (STJ11101713). Western blot was performed from the immunoprecipitate using [KO Validated] p53 Rabbit monoclonal antibody (STJ11101713) at a dilution of 1:1000.

Anti-TP53 antibody (1-393) [S3MR] (STJ11101713)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Rat

$197.78 - $867.38

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Western blot analysis of lysates from 1) p53 knockout A431 cell, 2) A431 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-TP53 antibody (10-59 aa) (STJ94893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Immunoprecipitation analysis of 200 Mu g extracts of U-251MG cells using 3 Mu g p53 antibody (STJ28328). Western blot was performed from the immunoprecipitate using p53 antibody (STJ28328) at a dilution of 1:1000.

Anti-TP53 antibody (218-390) (STJ28328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

$151.88 - $708.08

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Human esophageal squamous cell carcinoma tissue was stained with Anti-P53 (ABT-P53) Antibody

Anti-TP53 antibody (250-393 aa) [ABT-P53] (STJ197083)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$234.90 - $340.20

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Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-P53 (ABT002) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 53kDaObserved band size: 53kDa

Anti-TP53 antibody (250-393 aa) [ABT002] (STJ197180)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$234.90 - $340.20

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Anti-P53 antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-TP53 antibody (280aa C-Term) (STJ140047)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

$137.03 - $532.58

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Anti-P53 antibody at 1:2500 dilution; 50 µg of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-TP53 antibody (280aa C-Term) (STJ140114)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

$137.03 - $532.58

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Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TP53 antibody (283-332 aa) (STJ94905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of various cells using p53 Polyclonal Antibody diluted at 1：1000

Anti-TP53 antibody (301-350 aa) (STJ94894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha (phospho Ser51) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). p53 monoclonal antibody (6C4) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-TP53 antibody [6C4] (STJ96954)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-TP53 antibody [ABT239R] (STJ197050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human

$234.90 - $340.20

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Human breast carcinoma infiltrating fat stained with anti-p53 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of carcinoma cells.

Anti-TP53 antibody [DO‐7] (STJ180207)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$218.03 - $650.03

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Anti-TP53 antibody [Pab122] (STJ16100763)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Dog/Human/Monkey/Mouse/Rat

$388.13 - $1,788.08

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Western blot analysis of various lysates using p53 Mouse monoclonal antibody (STJ112620) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TP53 antibody [S0MM] (STJ112620)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis of lysates from 293T cells using [KO Validated] p53 Rabbit monoclonal antibody (STJ11105127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TP53 antibody [S5127RM] (STJ11105127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Western blot analysis of various cells using 53BP1 Polyclonal Antibody diluted at 1：2000

Anti-TP53BP1 antibody (1-50 aa) (STJ91389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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