Anti-POLA2 antibody (STJ190749)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-DNA polymerase alpha subunit B for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLA2 |
| Gene ID | 23649 |
| Uniprot ID | DPOA2_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | DPOA2 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Function | Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. |
| Protein Name | Dna Polymerase Alpha Subunit BDna Polymerase Alpha 70 Kda Subunit |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-174411Reactome: R-HSA-174430Reactome: R-HSA-68952Reactome: R-HSA-68962Reactome: R-HSA-69091Reactome: R-HSA-69166Reactome: R-HSA-69183Reactome: R-HSA-9710421 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Polymerase Alpha Subunit B antibodyAnti-Dna Polymerase Alpha 70 Kda Subunit antibodyAnti-POLA2 antibody |
Information sourced from Uniprot.org