Phospho Antibodies | St John's Laboratory

Anti-Phospho-SGK1-S422 antibody [P10D6] (STJA0019167)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$510.98 - $2,203.88

Choose Options

Anti-Phospho-SGK1-S422 antibody [SAA0301] (STJA0019166)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

$510.98 - $2,203.88

Choose Options

Western blot analysis of VEC HY926 cells using Phospho-SGK1 (S422) Polyclonal Antibody diluted at 1：500

Anti-Phospho-SGK1-Ser422 antibody (381-430 aa) (STJ90404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of 293 using p-SGK1 (S78) antibody. Antibody was diluted at 1:2000

Anti-Phospho-SGK1-Ser78 antibody (41-90 aa) (STJ90493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SGK3-Thr320 antibody (STJ196416)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SGO1-Ser14 antibody (1-50 aa) (STJ91267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-SGTA-Ser305 antibody (STJ194746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Anti-Phospho-SH-PTP2-Tyr542 antibody [PT1271R] (STJA0040132)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

Add to Cart The item has been added

Western blot analysis of various cells using Phospho-Shb (Y246) Polyclonal Antibody

Anti-Phospho-SHB-Tyr246 antibody (STJ90405)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SHC1-Ser239/240 antibody (STJ196417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-Phospho-SHC1-Ser36 antibody (STJ91235)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SHC1-Tyr317 antibody (STJ196418)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

Choose Options

Western blot analysis of various cells using Phospho-Shc (Y349) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-SHC1-Tyr349 antibody (315-364 aa) (STJ90738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western Blot analysis of 1 MCF-7 cell 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SHC1-Tyr349+Tyr350 antibody (STJA0010063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of various cells using Phospho-Shc (Y427) Polyclonal Antibody diluted at 1：500

Anti-Phospho-SHC1-Tyr427 antibody (393-442 aa) (STJ90739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-Phospho-SHC1-Y317 antibody (STJ11101141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from A-431 cells, using Phospho-SHC1-Y427 Rabbit pAb (STJ11102671) at 1:1000 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-SHC1-Y427 antibody (STJ11102671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from 293 cells using Phospho-SHC1-Y427 Rabbit pAb (STJ11107624). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-SHC1-Y427 antibody (STJ11107624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human

$174.83 - $867.38

Choose Options

Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SHC3-Tyr424 antibody (STJA0009985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SIK1-Thr175/163 antibody (STJA0009930)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western Blot analysis of 1 MCF-7 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SIK1-Thr182 antibody (STJA0009905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western Blot analysis of 1 Hela, 2 treated with LPS 100ng/mL 20mim, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-SIK2-Thr175/163 antibody (STJA0009906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of lysates from 293T cells, using Phospho-SIRT1-S47 Rabbit mAb (STJ11102569) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-SIRT1-S47 antibody [S9MR] (STJ11102569)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

Choose Options

Anti-Phospho-SIRT1-Ser27 antibody (STJ196424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Anti-Phospho-SIRT1-Ser47 antibody (STJ90648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

Choose Options

Anti-Phospho-SIRT1-Ser549 antibody (STJA0009874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

$64.80 - $340.20

Choose Options

Anti-Phospho-SKP2-Ser64 antibody (STJ196425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Anti-Phospho-SLAMF1-Tyr327 antibody (STJA0009978)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot of rat striatal lysate showing specific immunolabeling of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-SLC6A3-Thr53 antibody (STJA0003618)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat

$613.58

Choose Options

Western blot of rat mid brain membrane lysate showing specific immunolabeling of the ~76 kDa SERT protein phosphorylated at Thr276 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-Slc6a4-Thr276 antibody (STJA0003776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Bovine/Human/Non-Human Primates/Sheep/Xenopus

$613.58

Choose Options

Western blot analysis of lysates from C6 cells, using Phospho-Smad1-S206 Rabbit pAb (STJ22396) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-SMAD1-S206 antibody (STJ22396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Anti-Phospho-SMAD1-S463/S465+SMAD5-S463/S465+SMAD9-S465/S467-S463/S465+S463/S465+S465/S467 antibody [SRM15144] (STJ11109874)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

Add to Cart The item has been added

Western blot analysis of lysates from HeLa cells using Phospho-Smad1-S465 Rabbit pAb (STJ11107625). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Smad1-S465 antibody (STJ11107625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Western blot analysis of lysates from 293 cells and HepG2 cells, using Smad1 (Phospho-Ser187) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SMAD1-Ser187 antibody (153-202 aa) (STJ90904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMAD1-Ser206 antibody (STJ196428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

Choose Options

Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMAD1-Ser214 antibody (STJA0009831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-Phospho-SMAD1-Ser463+Ser465 antibody (STJA0009975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of various cells using Phospho-Smad1 (S465) Polyclonal Antibody

Anti-Phospho-SMAD1-Ser465 antibody (416-465 aa) (STJ90743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of extracts of various cell lines, using Phospho-Smad2-S465/467 + Smad3-S423/425 antibody (STJ11105832) at 1:600 dilution. C6 cells were treated by TGF-Beta 3 (200 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-Smad2 + Smad3-S465/S467-S423/S425 antibody [S5832RM] (STJ11105832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

Choose Options

Western blot analysis of various lysates, using Phospho-Smad2-S245/S250/S255 antibody (STJ11105827) at1:2000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-SMAD2-S245/S250/S255 antibody [S5827RM] (STJ11105827)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

Choose Options

Western blot analysis of various lysates using Phospho-Smad2-S250 Rabbit mAb (STJ11102595) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C2C12 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-SMAD2-S250 antibody [S5MR] (STJ11102595)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Smad2-S465/S467 Rabbit pAb (STJ11101059) at 1:1000 dilution. HeLa cells were treated by TGF-Beta (10ng/mL) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-SMAD2-S465/S467 antibody (STJ11101059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

Choose Options

Western blot analysis of extracts of various lysates, using Phospho-Smad2-S465/S467 antibody (STJ11105831) at1:2000 dilution. HeLa and NIH/3T3 were treated by TGF-Beta 3 (H) (200 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-SMAD2-S465/S467 antibody [S5831RM] (STJ11105831)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

Choose Options

Western blot analysis of lysates from HeLa cells, using Phospho-Smad2-S467 Rabbit pAb (STJ22397) at 1:1000 dilution. HeLa cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-SMAD2-S467 antibody (STJ22397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

Choose Options

Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMAD2-Ser245 antibody (STJA0009833)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30', using Smad2 (Phospho-Ser250) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-SMAD2-Ser250 antibody (216-265 aa) (STJ90521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options

Anti-Phospho-Smad2-Ser250 antibody [PT0058R] (STJA0039594)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$0.00

Add to Cart The item has been added

Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-SMAD2-Ser255 antibody (STJA0009832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

Choose Options