Phospho Antibodies | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ARHGAP31-Thr789 antibody (STJ91246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 293T cells using Phospho-GRF-1 (Y1087) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ARHGAP35-Tyr1087 antibody (STJ90281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 3T3 cells using Phospho-GRF-1 (Y1105) Polyclonal Antibody

Anti-Phospho-ARHGAP35-Tyr1105 antibody (1071-1120 aa) (STJ90795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-ARHGAP42-Tyr376 antibody (STJ196241)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using ARHGDIA (Phospho-Ser174) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ARHGDIA-Ser174 antibody (140-189 aa) (STJ90922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of C6 cells, using Phospho-ARHGEF2-S886 antibody (STJ11101060) at 1:1000 dilution. C6 were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-ARHGEF2-S886 antibody (STJ11101060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ARHGEF2-Ser885 antibody (851-900 aa) (STJ90558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using AurB/C (Phospho-Thr236/202) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ARK-2/3-Thr236/202 antibody (201-250 aa) (STJ91175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-ARPC1B-Thr-21 antibody (STJA0005270)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$493.43

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Anti-Phospho-ARRB1-Ser412 antibody (369-418 aa) (STJ90802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

$64.80 - $340.20

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Western blot analysis of HeLa, using Phospho-ATF-2 (Thr69/71) /ATF-7 (Thr51/53) Rabbit polyclonal antibody (STJ11105884) at 1:600 dilution. HeLa cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-ATF-2/ATF-7-Thr69/71-Thr51/53 antibody (STJ11105884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of Mouse-kidney KB 293T 3T3 lysis using Phospho-ATF-1 (S63) antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-ATF1-Ser63 antibody (31-80 aa) (STJ90454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-ATF-2 (S112) Polyclonal Antibody diluted at 1：500

Anti-Phospho-ATF2-Ser112 antibody (79-128 aa) (STJ90183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using ATF-2 (Phospho-Ser472) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATF2-Ser472 antibody (441-490 aa) (STJ91240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ATF2 (Phospho-Ser480) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATF2-Ser480 antibody (456-505 aa) (STJ91069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat

$64.80 - $340.20

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Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74 kDa ATF2 protein phosphorylated at Ser490 and Ser498.

Anti-Phospho-ATF2-Ser490/498 antibody (STJA0003583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

$613.58

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Western blot analysis of various cells using Phospho-ATF-2 (S62) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ATF2-Ser62 antibody (29-78 aa) (STJ90184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 polyclonal antibody (STJ11101048) at 1:1000 dilution or ATF2 antibody (A2155). 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-ATF2-T69/71 antibody (STJ11101048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of 293T cells, using Phospho-ATF2-T69/T71 antibody (STJ110861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit.

Anti-Phospho-ATF2-T69/T71 antibody (STJ110861)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of various cell lines, using Phospho-ATF2-T71 antibody (STJ22025) at 1:1000 dilution. HeLa cells were treated by Anisomycin (25ug/mL) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25ug/mL) for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-ATF2-T71 antibody (STJ22025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ATF2-T71 rabbit monoclonal antibody (STJ11102621) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 ug/mL) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Phospho-ATF2-T71 antibody [S1MR] (STJ11102621)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$197.78 - $867.38

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Western blot of UACC903 melanoma cell line lysate (DMSO) showing specific immunolabeling of the ~ 72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Gö6850. Image courtesy of Eric Lau and Ze NA ev Ronai.

Anti-Phospho-ATF2-Thr52 antibody (STJA0003584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$613.58

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ATF2 (Phospho-Thr69 or 51) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATF2-Thr69 antibody (36-85 aa) (STJ90185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-ATF2-Thr69/71 antibody (STJ196245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-ATF-2 (T71) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-ATF2-Thr71 antibody (38-87 aa) (STJ90186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using Phospho-ATF-2 (T73) Polyclonal Antibody

Anti-Phospho-ATF2-Thr73 antibody (40-89 aa) (STJ90187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts from HeLa cells using Phospho-ATF4-S245 antibody (STJ22027). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-ATF4-S245 antibody (STJ22027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-ATG13-Ser355 antibody (STJ11101125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-ATG13-Ser355 antibody (STJ196246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Phospho-ATG14-Ser29 antibody (STJ196247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using (STJ22028) at 1:1000 dilution. 293T cells were treated by Doxorubicin (0. 5 uM) at 37 °C for 24 hours. 293T cells were treated by UV at room temperature for 15-30 minutes. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-ATM-S1981 antibody (STJ22028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Western blot analysis of extracts of 293T cells, using Phospho-ATM-S1981 rabbit monoclonal antibody (STJ11102610) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Phospho-ATM-S1981 antibody [S0MR] (STJ11102610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$197.78 - $867.38

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Anti-Phospho-ATM-Ser-1981 antibody [M366] (STJA0005274)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Mouse/Rat

$493.43

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Anti-Phospho-ATM-Ser-794 antibody (STJA0005273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/FC

Reactivity :

Human/Mouse/Rat

$493.43

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Western Blot analysis of 1 K562 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser179 antibody (STJA0010053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of K562 using p-Atm (S1981) antibody. Antibody was diluted at 1:500

Anti-Phospho-ATM-Ser1981 antibody (STJ97691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-ATM-Ser1987 antibody (STJA0009856)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western Blot analysis of mouse heart, Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser367 antibody (STJA0010035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of 293T cells, using Phospho-Na+/K+-ATPase-S16 antibody (STJ11101103) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-ATP1A1-S16 antibody (STJ11101103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ATPase (Phospho-Ser16) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATP1A1-Ser16 antibody (5-54 aa) (STJ90804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of KB Hela lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-ATP1A1-Tyr260 antibody (230-290 aa) (STJ98679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-ATP1A1-Tyr260 antibody (STJ99603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of 293T, using Phospho-ATR-S428 antibody (STJ114848) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody (STJ114848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of various lysates, using Phospho-ATR-S428 antibody (STJ11105842) at1:2000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody [S5842RM] (STJ11105842)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATR (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATR-Ser428 antibody (394-443 aa) (STJ91234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of HeLa cells, using Phospho-ATR-T1989 antibody (STJ11103765) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Phospho-ATR-T1989 antibody (STJ11103765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-Phospho-ATR-Thr1989 antibody (STJ196248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATRIP (Phospho-Ser224) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATRIP-Ser224 antibody (190-239 aa) (STJ90973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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