Anti-Phospho-AQP2-Ser264 antibody (STJA0003581)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic phospho-peptide corresponding to amino acid residues surrounding Ser264 conjugated to KLH.
STJA0003581-100
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General Information

Short DescriptionRabbit polyclonal anti-Phospho-Aquaporin 2-Ser264 for use in WB and IHC in Human, Mouse, Rat, Bovine, Canine, Chicken and Non-Human Primates samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC
HostRabbit
ReactivityHuman/Mouse/Rat/Bovine/Canine/Chicken/Non-Human Primates
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
PurificationThis antibody was antigen affinity purified from pooled serum.
Dilution RangeWB 1:1000
IHC 1:100
Formulation100 ยตl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 ยตg per ml BSA and 50% Glycerol.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolAqp2
Gene ID25386
Uniprot IDAQP2_RAT
ImmunogenSynthetic phospho-peptide corresponding to amino acid residues surrounding Ser264 conjugated to KLH.

Additional Info

Tissue Specificity Detected in kidney, in cortical and the medullary collecting tubules (at protein level). Detected in kidney medulla and cortex.
Post Translational Modifications Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. N-glycosylated.
Function Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis. Could also be permeable to glycerol.
Protein Name Aquaporin-2
Aqp-2
Adh Water Channel
Aquaporin-Cd
Aqp-Cd
Collecting Duct Water Channel Protein
Wch-Cd
Water Channel Protein For Renal Collecting Duct
Database Links Reactome: R-RNO-432040
Reactome: -RNO-432047
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Cell Membrane
Cytoplasmic Vesicle Membrane
Golgi Apparatus
Trans-Golgi Network Membrane
Shuttles From Vesicles To The Apical Membrane
Vasopressin-Regulated Phosphorylation Is Required For Translocation To The Apical Cell Membrane
Plekha8/Fapp2 Is Required To Transport Aqp2 From The Tgn To Sites Where Aqp2 Is Phosphorylated
Alternative Antibody Names Anti-Aquaporin-2 antibody
Anti-Aqp-2 antibody
Anti-Adh Water Channel antibody
Anti-Aquaporin-Cd antibody
Anti-Aqp-Cd antibody
Anti-Collecting Duct Water Channel Protein antibody
Anti-Wch-Cd antibody
Anti-Water Channel Protein For Renal Collecting Duct antibody
Anti-Aqp2 antibody

Information sourced from Uniprot.org

Citations

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