Western blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit monoclonal antibody (STJ11105892) at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-AMPKalpha1+AMPKalpha2-T183-T172 antibody [S5892RM] (STJ11105892)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11105892
🚚 Free UK Delivery on orders over £150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionRabbit monoclonal Phospho-AMPKalpha1+AMPKalpha2-T183-T172 antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS5892RM
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:5000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPRKAA2
PRKAA1
Gene ID5563
5562
Uniprot IDAAPK2_HUMAN
AAPK1_HUMAN
Immunogen SequenceLRTSC
SpecificityA synthetic phosphorylated peptide around T183/T172 of human PRKAA1/PRKAA2 (NP_006242.5/NP_006243.2).

Additional Info

Tissue Specificity
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-2 antibody
Anti-Ampk Subunit Alpha-2 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-PRKAA2 antibody
Anti-AMPK antibody
Anti-AMPK2 antibody
Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-1 antibody
Anti-Ampk Subunit Alpha-1 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-Tau-Protein Kinase Prkaa1 antibody
Anti-PRKAA1 antibody
Anti-AMPK1 antibody

Information sourced from Uniprot.org

Citations

Product Review

!
Well-cited Academic Publications
KO-Validated 700+ Lines
Technical Support Expert Scientific Team
1-Year Guarantee Antibodies & Proteins