Rabbit Monoclonal Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Prohibitin antibody (STJ11101679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PHB antibody (100-200) [S9MR] (STJ11101679)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using Prohibitin 2 (Prohibitin 2 (PHB2) ) Rabbit mAb (STJ11102436) at 1:5000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-PHB2 antibody (100-200) [S6MR] (STJ11102436)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-PHD3 antibody [SRM14447] (STJ11109177)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of extracts from wild type (WT) and PHF11 knockdown (KD) 293T cells, using PHF11 Rabbit monoclonal antibody (STJ11105273) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PHF11 antibody (40-321) [S5273RM] (STJ11105273)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using PHF8 antibody (STJ11103129) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PHF8 antibody (800-900) [S9MR] (STJ11103129)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-PHGDH antibody [SRM14489] (STJ11109219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Human tonsil stained with anti-PHH3 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of mitotic lymphocytes.

Anti-PHH3 antibody (1-100aa) [ZR285] (STJ180477)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

€201.88 - €703.13

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Anti-Phospho-4E-BP1 )-Thr37/46 antibody [PT0862R] (STJA0039150)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€356.25 - €675.00

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Anti-Phospho-4E-BP1-Thr70 antibody [PT1203R] (STJA0040179)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-ABL1-Tyr245 antibody [PT1402R] (STJA0040363)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

€187.50 - €675.00

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Anti-Phospho-ACC1/ACC2-S79 antibody [SRM5539] (STJ11107725)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€179.38 - €773.13

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Anti-Phospho-Acetyl CoA Carboxylase 1-S79-S79 antibody [SRM15134] (STJ11109864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of NIH/3T3, using Phospho-Acetyl CoA Carboxylase-S79 antibody (STJ11105862) at 1:7000 dilution. NIH/3T3 cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-Acetyl CoA Carboxylase-S79 antibody [S5862RM] (STJ11105862)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€179.38 - €773.13

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Anti-Phospho-Acetyl-CoA Carboxylase-Ser79 antibody [PT0983R] (STJA0039832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€356.25 - €675.00

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Anti-Phospho-ACLY-S455-S455 antibody [SRM15137] (STJ11109867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-Akt-Ser473 antibody [PT0470R] (STJA0039543)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Western blot analysis of various lysates using Phospho-Akt-T308 Rabbit mAb (STJ11103774) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-Akt-T308 antibody [S4MR] (STJ11103774)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€179.38 - €773.13

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Anti-Phospho-Akt-Thr308 antibody [PT0722R] (STJA0039210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using Phospho-Akt-S473 Rabbit mAb (STJ11103745) at 1:1000 dilution. Both Jurkat and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1 & AKT2 & AKT3-S473 & S474 & S472 antibody [S5MR] (STJ11103745)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S124 Rabbit mAb (STJ11102574) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S124 antibody [S4MR] (STJ11102574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S129 Rabbit mAb (STJ11102573) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S129 antibody [S3MR] (STJ11102573)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of various lysates using Phospho-AKT1-S473 mAb (STJ113518) at 1:1000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody [S8MR] (STJ113518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of paraffin-embedded human kidney using Phospho-Akt-T308 Rabbit monoclonal antibody (STJ11105823) at dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-AKT1-T308 antibody [S5823RM] (STJ11105823)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-AKT1-T450 + AKT2-T451 + AKT3-T447-T450+T451+T447 antibody [SRM15129] (STJ11109859)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from C6 cells, using Phospho-AKT1-T450 Rabbit mAb (STJ11102572) at 1:1000 dilution. C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by PDGF (50 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-T450 antibody [S2MR] (STJ11102572)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

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Western blot analysis of lysates from HeLa cells using Phospho-AKT1S1/PRAS40-T246 Rabbit mAb (STJ11107731) at 1:8100 dilution incubated overnight at 4C. HeLa cells were treated by Insulin (50 nM) at 37C for 5 minutes after serum-starvation overnight. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br> Blocking buffer: 3% nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 60s.

Anti-Phospho-AKT1S1/PRAS40-T246 antibody [SRM5542] (STJ11107731)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Anti-Phospho-AKT2-S474 antibody [SRM13696] (STJ11108426)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Anti-Phospho-AMPK Alpha 1-Ser485 antibody [PT1374R] (STJA0040378)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-AMPK Alpha 1/2-Thr183/Thr172 antibody [PT0920R] (STJA0039809)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit monoclonal antibody (STJ11105892) at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-AMPKalpha1+AMPKalpha2-T183-T172 antibody [S5892RM] (STJ11105892)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-ASK1-T845-T845 antibody [SRM15151] (STJ11109881)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Mouse

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T71 Rabbit mAb (STJ11102621) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-ATF2-T71 antibody [S1MR] (STJ11102621)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

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Confocal imaging of paraffin-embedded Human colon tissue (untreated) and Human colon tissue (treated with Lambda pp) using Phospho-ATM-S1981 Rabbit mAb (STJ11102610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-Phospho-ATM-S1981 antibody [S0MR] (STJ11102610)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

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Anti-Phospho-ATM-Ser1981 antibody [PT0902R] (STJA0039795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates, using Phospho-ATR-S428 antibody (STJ11105842) at1:2000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody [S5842RM] (STJ11105842)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Anti-Phospho-ATR-S428-S428 antibody [SRM15157] (STJ11109887)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human

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Anti-Phospho-ATR-Ser428 antibody [PT1243R] (STJA0040115)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of A-549, using Phospho-Axl-Y702 Rabbit monoclonal antibody (STJ11105882) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Phospho-AXL-Y702 antibody [S5882RM] (STJ11105882)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb (STJ11102596) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight or treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-BAD-S112 antibody [S6MR] (STJ11102596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Anti-Phospho-Bad-S112 antibody [SRM13686] (STJ11108416)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-Bcl-2-Ser70 antibody [PT1385R] (STJA0040038)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human

€187.50 - €675.00

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Anti-Phospho-Beclin 1-S90 antibody [SRM5546] (STJ11107736)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-Phospho-Beta Catenin-Ser37 antibody [PT0894R] (STJA0039787)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€187.50 - €675.00

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Anti-Phospho-Beta Catenin-Ser675 antibody [PT0838R] (STJA0039149)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€356.25 - €675.00

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Anti-Phospho-Beta Catenin-Thr41/Ser45 antibody [PT0637R] (STJA0039323)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb (STJ11102615) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-BRAF-T401 antibody [S5MR] (STJ11102615)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

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Western blot analysis of various lysates using Phospho-BRD4-S1083 Rabbit mAb (STJ11103761) at1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. HeLa cells were treated by nocodazole (50 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 300s.

Anti-Phospho-BRD4-S1083 antibody [S1MR] (STJ11103761)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Western blot analysis of various lysates using (STJ11103782) at1:1000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-BRD4-T204 antibody [S2MR] (STJ11103782)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€148.13 - €651.88

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