Western Blot Antibodies | St John's Laboratory

Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody (STJ22761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-BAX antibody (1-100) (STJ22761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Immunohistochemistry analysis of Bax in paraffin-embedded human colon carcinoma using Bax Rabbit monoclonal antibody (STJ11101764) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-BAX antibody (1-100) [S4MR] (STJ11101764)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of extracts of various cell lines, using Bax antibody (STJ118092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-BAX antibody (1-211) (STJ118092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of L929 cells using Bax Rabbit polyclonal antibody (STJ29763) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BAX antibody (1-211) (STJ29763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of lysates from 1) Hela, 2) MCF7, 3) k562, 4) A549 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Tubulin Beta monoclonal antibody (5G3) (cat: (STJ96932) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-BAX antibody (1-50 aa) (STJ91824)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates, using Bax antibody (STJ11103230) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Bax antibody (10-70) [S0MR] (STJ11103230)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Immunohistochemical analysis of paraffin-embedded Rat Testis Tissue using Bax Mouse mAb diluted at 1:200.

Anti-BAX antibody (30-110 aa) [1C1] (STJ97729)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from 1) Hela Cell Lysate, 2) C2C12 Cell Lysate, 3) PC12 Cell Lysate cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-BAX antibody (30-110 aa) [6F11] (STJ97725)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC

Reactivity :

Human/Rat/Mouse/Chicken/Silkworm

€60.00 - €315.00

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Western blot analysis of lysates from 1) Hela, 2) MCF-7, 3) 3T3 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） GAPDH monoclonal antibody (2B8) (cat: (STJ96931) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-BAX antibody (80-129 aa) (STJ91820)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

€60.00 - €315.00

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Anti-BAX antibody at 1:2500 dilution 50 µg of total protein per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-BAX antibody (90aa N-Term) (STJ140113)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

€126.88 - €493.13

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Western blot detection of Bax in COS7, Raji, CEM and Hela cell lysates using Bax mouse mAb (1:1000 diluted). Predicted band size:20KDa. Observed band size:20KDa.

Anti-BAX antibody [2B12-G6-H6] (STJ99209)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Monkey

€180.00 - €435.00

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Anti-BAX antibody [3C10] (STJA0022337)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human

€473.13 - €2,040.63

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Anti-BAX antibody [6A7] (STJA0019665)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC/WB

Reactivity :

Human/Mouse

€426.88 - €1,809.38

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Western blot analysis of Bax at dilution of 1:1000

Anti-Bax antibody [SM5115] (STJA0005115)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

€213.13 - €291.88

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Western blot analysis of various lysates using BAZ1A Rabbit pAb (STJ111908) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-BAZ1A antibody (1347-1556) (STJ111908)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from HuvEc cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BAZ1A antibody (736-786 aa) (STJ195028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates using WSTF Rabbit mAb (STJ11102480) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-BAZ1B antibody (1-100) [S0MR] (STJ11102480)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Western blot analysis of various lysates using [KO Validated] WSTF Rabbit pAb (STJ111893) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-BAZ1B antibody (1-260) (STJ111893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various lysates using WSTF Rabbit pAb (STJ114419) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-BAZ1B antibody (1-260) (STJ114419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of the lysates from HeLa cells using BAZ2A antibody.

Anti-BAZ2A antibody (1281-1330 aa) (STJ96028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates using BAZ2A/ TIP5 Rabbit polyclonal antibody (STJ119688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-BAZ2A antibody (97-295) (STJ119688)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunofluorescence analysis of U-2 OS cells using BAZ2A Rabbit polyclonal antibody (STJ119689) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BAZ2A antibody (97-295) (STJ119689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Confocal imaging of U-2 OS cells using PUMA Rabbit monoclonal antibody (STJ11101945, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-BBC3 antibody (100-193) [S5MR] (STJ11101945)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Western blot analysis of various cells using PUMA Polyclonal Antibody

Anti-BBC3 antibody (120-169 aa) (STJ95272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of NIH-3T3 cells using PUMA Rabbit polyclonal antibody (STJ119319) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-BBC3 antibody (50-131) (STJ119319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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STJ72378 (0. 1µg/ml) staining of Peripheral Blood Lymphocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BBC3 antibody (Internal) (STJ72378)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

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Anti-BBC3 antibody (STJA0005744)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€569.38

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Anti-BBC3 antibody (STJA0009776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€60.00 - €315.00

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Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BBOF1 antibody (124-174 aa) (STJ194591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using BBOX1 antibody (STJ28373) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-BBOX1 antibody (1-180) (STJ28373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-BBOX1 antibody (STJA0009073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-BBS1 antibody (STJA0008186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€60.00 - €315.00

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Anti-BBS1 antibody [N292/13] (STJA0021204)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Anti-BBS1 antibody [N292/15] (STJA0021205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Anti-BBS1 antibody [N292/49] (STJA0021206)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Western blot analysis of various lysates using BBS10 Rabbit pAb (STJ111434) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-BBS10 antibody (474-723) (STJ111434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Anti-BBS10 antibody (STJA0007443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using BBS2 antibody (STJ29561) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.

Anti-BBS2 antibody (1-96) (STJ29561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-BBS2 antibody (149-199 aa) (STJ194990)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-BBS2 antibody (N-Term) (STJ13100735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

€414.38

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Anti-BBS3/Arl6 antibody [N302/10] (STJA0021364)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Anti-BBS3/Arl6 antibody [N302/46] (STJA0021365)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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STJ72458 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BBS4 antibody (125-139) (STJ72458)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

€390.63

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STJ72457 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-BBS4 antibody (33-46) (STJ72457)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Dog/Pig

€390.63

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Western blot analysis of lysates from NCI-H460 cells, using BBS4 Rabbit pAb (STJ117805) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-BBS4 antibody (350-519) (STJ117805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-BBS4 antibody (N-Term) (STJ13100736)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

€414.38

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Anti-BBS4 antibody (STJA0006876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from 293T cells, using BBS5 Rabbit pAb (STJ114554) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 10s.

Anti-BBS5 antibody (1-341) (STJ114554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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