• Western blot analysis of extracts of various cell lines, using ATOH1 Antibody (STJ28613) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ATOH1 antibody (215-354) (STJ28613)

SKU:
STJ28613

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ATOH1 (215-354) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATOH1
Gene ID: 474
Uniprot ID: ATOH1_HUMAN
Immunogen Region: 215-354
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human ATOH1 (NP_005163.1).
Immunogen Sequence: LQTPSGGEQPPPPPASCKSD HHHLRTAASYEGGAGNATAA GAQQASGGSQRPTPPGSCRT RFSAPASAGGYSVQLDALHF STFEDSALTAMMAQKNLSPS LPGSILQPVQEENSKTSPRS HRSDGEFSPHSHYSDSDEAS
Function Transcriptional regulator. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. Plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.
Protein Name Transcription Factor Atoh1
Atonal Bhlh Transcription Factor 1
Class A Basic Helix-Loop-Helix Protein 14
Bhlha14
Helix-Loop-Helix Protein Hath-1
Hath1
Protein Atonal Homolog 1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor Atoh1 antibody
Anti-Atonal Bhlh Transcription Factor 1 antibody
Anti-Class A Basic Helix-Loop-Helix Protein 14 antibody
Anti-Bhlha14 antibody
Anti-Helix-Loop-Helix Protein Hath-1 antibody
Anti-Hath1 antibody
Anti-Protein Atonal Homolog 1 antibody
Anti-ATOH1 antibody
Anti-ATH1 antibody
Anti-BHLHA14 antibody

Information sourced from Uniprot.org

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