Anti-ATN1 antibody (81-130 aa) (STJ91784)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human ATN1 at the amino acid range 81-130
General Information
| Short Description | Rabbit polyclonal anti-Atrophin-1 (81-130 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ATN1 |
| Gene ID | 1822 |
| Uniprot ID | ATN1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human ATN1 at the amino acid range 81-130 |
| Immunogen Region | 81-130 aa |
| Specificity | Atrophin-1 Polyclonal Antibody detects endogenous levels of Atrophin-1 protein. |
Additional Info
| Post Translational Modifications | Phosphorylated in vitro by MAPK8/JNK1 on Ser-739. Mutant ATN1 sequences with expanded poly-Gln (polyQ) traits are more slowly phosphorylated. Proteolytically cleaved, probably in the nucleus, to produce two C-terminal fragments of 140 kDa (F1) and 125 kDa (F2) each containing poly-Gln (polyQ) tracts. F2 is produced by cleavage by caspases and is exported into the cytoplasm. In vitro, cleavage increases with an increase in the number of polyQ tracts. C-terminal proteolytic products appear to be the cause of cell toxicity. In vitro cleavage at Asp-109. |
| Function | Transcriptional corepressor. Recruits NR2E1 to repress transcription. Promotes vascular smooth cell (VSMC) migration and orientation. Corepressor of MTG8 transcriptional repression. Has some intrinsic repression activity which is independent of the number of poly-Gln (polyQ) repeats. |
| Protein Name | Atrophin-1Dentatorubral-Pallidoluysian Atrophy Protein |
| Database Links | Reactome: R-HSA-8943724 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionCell JunctionShuttles Between Nucleus And CytoplasmColocalizes With Fat1 In The Perinuclear AreaAt Cell-Cell Junctions And Leading Edges Of CellsColocalizes With Mtg8 In Discrete Nuclear DotsProteolytic Fragment F1 Appears To Remain In NucleusFragment F2 Is Exported Into The CytoplasmFragment F2 From Mutant Sequences With Longer Poly-Gln (Polyq) Tracts Are Additionally Located To The Cytoplasmic Membrane And To Certain Organelles |
| Alternative Antibody Names | Anti-Atrophin-1 antibodyAnti-Dentatorubral-Pallidoluysian Atrophy Protein antibodyAnti-ATN1 antibodyAnti-D12S755E antibodyAnti-DRPLA antibody |
Information sourced from Uniprot.org