Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from rat brain, using ATG4B Rabbit pAb (STJ116047) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-ATG4B antibody (1-393) (STJ116047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using ATG4B antibody (STJ22639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-ATG4B antibody (1-393) (STJ22639)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-ATG4B antibody (20-80 aa) (STJ13100547)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Western blot analysis of Jurkat cells using Atg4b Polyclonal Antibody

Anti-ATG4B antibody (71-120 aa) (STJ91759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-ATG4B antibody (STJ13100552)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Western blot analysis of extracts of various cell lines, using ATG4C antibody (STJ29533) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATG4C antibody (1-200) (STJ29533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ATG4C antibody (21-70 aa) (STJ91760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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STJ71866 (2µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ATG4C antibody (C-Term) (STJ71866)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Dog/Cow

£312.50

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Anti-ATG4C antibody (STJ13100529)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-ATG4C antibody (STJ13100536)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-ATG4C antibody (STJ13100540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Western blot analysis of lysates from wild type (WT) and ATG4D knockout (KO) HeLa cells, using [KO Validated] ATG4D Rabbit polyclonal antibody (STJ11100070) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ATG4D antibody (1-230) (STJ11100070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of U2OS cells using ATG4D Rabbit polyclonal antibody (STJ11104482) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATG4D antibody (1-230) (STJ11104482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-ATG4D antibody (10-50 aa) (STJ13100546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of lysates from Rat skeletal muscle using ATG4D Rabbit pAb (STJ110152) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ATG4D antibody (265-474) (STJ110152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Western blot analysis of Colo using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ATG4D antibody (Internal) (STJ91761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-ATG4D antibody (STJ13100545)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse

£331.50

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Anti-ATG4D antibody (STJ13100549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Anti-ATG4D antibody (STJ13100550)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-ATG4D antibody (STJ13100551)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Immunoprecipitation analysis of 300 Mu g extracts from 293T cells using 3 Mu g [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759). Western blot was performed from the immunoprecipitate using [KO Validated] ATG5 Rabbit monoclonal antibody (STJ11101759) at a dilution of 1:1000.

Anti-ATG5 antibody (1-100) [S9MR] (STJ11101759)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of K-562 cells using ATG5 Rabbit polyclonal antibody (STJ22711) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATG5 antibody (1-275) (STJ22711)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ATG5 antibody (180-260) (STJA0010105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ATG5 antibody (230-269 aa) (STJ13100561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

D.melanogaster

£331.50

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Immunohistochemistry analysis of paraffin-embedded mouse pancreas using ATG5 antibody (STJ113131) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ATG5 antibody (28-275) (STJ113131)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ATG5 antibody (N-Term) (STJ13100543)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

D.melanogaster

£331.50

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Anti-ATG5 antibody (STJ13100001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£331.50

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Anti-ATG5 antibody (STJ13100003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Anti-ATG5 antibody (STJ13100542)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-ATG5 antibody (STJ13100577)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse

£331.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-ATG5 antibody (STJ197434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, CREB-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). ATG5 mouse monoclonal antibody (3C7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ATG5 antibody (STJ98903)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/WB/IHC/IP

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ATG5 antibody [PT0514R] (STJA0039479)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemistry analysis of ATG7 in paraffin-embedded human esophageal cancer using [KD Validated] ATG7 Rabbit monoclonal antibody (STJ11101724) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ATG7 antibody (1-100) [S4MR] (STJ11101724)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-ATG7 antibody (521-570) (STJA0010166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ATG7 antibody (C-Term) (STJ13100000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-ATG7 antibody (C-Term) (STJ13100532)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-ATG7 antibody (C-Term) (STJ13100535)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-ATG7 antibody (N-Term) (STJ13100599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-ATG7 antibody (STJ197433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ATG7 antibody [PT0335R] (STJA0039604)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of lysates from Zea mays, using ATG8 Rabbit polyclonal antibody (STJ11104795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ATG8 antibody (1-100 aa) (STJ11104795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana/Z.mays/T.aestivum

£146.50 - £642.50

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Anti-ATG8E antibody (STJ11106778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

O.sativa

£146.50 - £642.50

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Anti-ATG9 antibody (Internal Region) (STJ13100562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

D.melanogaster

£331.50

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Western blot analysis of lysates from Mouse liver, using ATG9A Rabbit pAb (STJ110301) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 5s.

Anti-ATG9A antibody (180-290) (STJ110301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ATG9A antibody (C-Term) (STJ13100025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Anti-ATG9A antibody (STJ13100538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£331.50

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Western blot analysis of 293T 3T3 cells using Atg9a Polyclonal Antibody diluted at 1:800. Secondary antibody was diluted at 1:20000

Anti-ATG9A antibody (STJ98589)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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