Western Blot Antibodies | St John's Laboratory

Western blot analysis of various lysates using ASIC5 Rabbit pAb (STJ112345) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ASIC5 antibody (140-330) (STJ112345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

$151.88 - $708.08

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Anti-ASIC5 antibody (2-50 aa) (STJ13100716)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse

$447.53

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASIC5 antibody (412-462 aa) (STJ193822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-ASIC5 antibody (455-505 aa) (STJ13100576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

$447.53

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Anti-ASL antibody (289-464) (STJ28440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ASL antibody (STJA0007584)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates, using ASMT rabbit polyclonal antibody (STJ28612) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ASMT antibody (1-298) (STJ28612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from 293 cells, using ASMT Rabbit polyclonal antibody (STJ113419). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ASMT antibody (76-298) (STJ113419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunoprecipitation analysis of 200 Mu g extracts of SW620 cells using 1 Mu g ASMTL antibody (STJ29101). Western blot was performed from the immunoprecipitate using ASMTL antibody (STJ29101) at a dilution of 1:500.

Anti-ASMTL antibody (1-290) (STJ29101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-ASMTL antibody (STJA0009684)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

$64.80 - $340.20

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Immunofluorescence analysis of NIH/3T3 cells using ASNA1 Rabbit polyclonal antibody (STJ22700) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ASNA1 antibody (1-348) (STJ22700)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot with Anti-ASNA1 Antibody (STJ500177) with peptide on at 1:500 dilution in buffer.

Anti-ASNA1 antibody (300-348) (STJ500177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-ASNA1 antibody (300-348) {Biotin} (STJ500178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-ASNA1 antibody (300-348) {FITC} (STJ500179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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STJ71537 (1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASNA1 antibody (Internal) (STJ71537)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Cow

$421.88

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Immunofluorescence analysis of U2OS cells using ASNS Rabbit polyclonal antibody (STJ27504) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ASNS antibody (212-561) (STJ27504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of ASNS in paraffin-embedded rat ovary using ASNS Rabbit polyclonal antibody (STJ118475) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ASNS antibody (262-561) (STJ118475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASNS antibody (390-440 aa) (STJ193544)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of MCF7 cells, using ASNS Rabbit monoclonal antibody (STJ11101267) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ASNS antibody (450-550) [S7MR] (STJ11101267)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Anti-ASNSD1 antibody (549-599 aa) (STJ193878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human ASNSD1 with DYKDDDDK tag probed with STJ72360 (1ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/10000) in lane C. Mock-transfected HEK293 probed with STJ72360 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-ASNSD1 antibody (Internal) (STJ72360)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB-Trf

Reactivity :

Human

$421.88

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Immunofluorescence analysis of MCF-7 cells using ASPA antibody (STJ29410). Blue: DAPI for nuclear staining.

Anti-ASPA antibody (1-313) (STJ29410)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from 3T3 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASPA antibody (67-117 aa) (STJ194160)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ72496 (0. 01µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Aspa-Mouse antibody (C-Term) (STJ72496)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse/Rat

$421.88

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Anti-Aspartate Aminotransferase antibody (STJ13100719)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human

$447.53

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Anti-Aspartate Aminotransferase antibody (STJ13100720)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human

$447.53

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STJ73458 (0. 3µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Aspartyl-tRNA Synthetase antibody (N-Term) (STJ73458)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse

$421.88

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Anti-ASPG antibody (112-162 aa) (STJ197782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using ASPH Rabbit polyclonal antibody (STJ115119) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ASPH antibody (111-270) (STJ115119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-ASPH antibody (STJA0010213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of L929 cells using ASPM Rabbit polyclonal antibody (STJ11100117) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ASPM antibody (450-550) (STJ11100117)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using ASPN Rabbit polyclonal antibody (STJ112349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ASPN antibody (100-379) (STJ112349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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STJ70669 (0. 1µg/ml) staining of Human Tonsil (A) , (0. 3ug/ml) Human Uterus (B) , Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Asporin/ASPN antibody (Internal) (STJ70669)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Rat

$421.88

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Anti-ASPRV1 antibody (STJA0009310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells, using 3 Mu g TUG/ASPSCR1 antibody (STJ29617). Western blot was performed from the immunoprecipitate using TUG/ASPSCR1 antibody (STJ29617) at a dilution of 1:1000.

Anti-ASPSCR1 antibody (284-553) (STJ29617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from CACO2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASPSCR1 antibody (327-377 aa) (STJ195050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASRGL1 antibody (57-107 aa) (STJ194694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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STJ71356 (0. 5µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASRGL1/ALP antibody (Internal) (STJ71356)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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Western blot analysis of various lysates using ASS1 Rabbit mAb (STJ11102420) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-ASS1 antibody (1-100) [S0MR] (STJ11102420)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of NIH/3T3 cells using ASS1 Rabbit polyclonal antibody (STJ117795) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ASS1 antibody (1-412) (STJ117795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ASS1 antibody (STJA0006817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-ASTE1 antibody (STJA0004442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

$189.68 - $560.93

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Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASTL antibody (115-165 aa) (STJ194863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from Raji cells, using ASTL Rabbit pAb (STJ117183) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-ASTL antibody (24-431) (STJ117183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-ASTN2 antibody (STJA0008822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-ASXL1 antibody (1120-1180) (STJ506146)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse/Rat

$577.13

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Anti-ASXL1 antibody (1120-1180) {Biotin} (STJ506152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse/Rat

$713.48

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Anti-ASXL1 antibody (1120-1180) {FITC} (STJ506153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Mouse/Rat

$713.48

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