• Immunofluorescence analysis of C6 cells using ARCN1 Rabbit polyclonal antibody (STJ117191) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ARCN1 Rabbit polyclonal antibody (STJ117191) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ARCN1 Rabbit polyclonal antibody (STJ117191) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ARCN1 Rabbit polyclonal antibody (STJ117191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ARCN1 antibody (212-511) (STJ117191)

SKU:
STJ117191

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ARCN1 (212-511) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARCN1
Gene ID: 372
Uniprot ID: COPD_HUMAN
Immunogen Region: 212-511
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 212-511 of human ARCN1 (NP_001646.2).
Immunogen Sequence: VAPAPARPSGPSKALKLGAK GKEVDNFVDKLKSEGETIMS SSMGKRTSEATKMHAPPINM ESVHMKIEEKITLTCGRDGG LQNMELHGMIMLRISDDKYG RIRLHVENEDKKGVQLQTHP NVDKKLFTAESLIGLKNPEK SFPVNSDVGVLKWRLQTTEE SFIPLTINCWPSESGNGCDV NIEYELQEDNLELNDVVITI PLPSGVGAPVIGEIDGEYRH DSRRNTLEWCLPVIDAKNK
Tissue Specificity Ubiquitously expressed.
Function Component of the coatomer, a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins.the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
Protein Name Coatomer Subunit Delta
Archain
Delta-Coat Protein
Delta-Cop
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle
Copi-Coated Vesicle Membrane
The Coatomer Is Cytoplasmic Or Polymerized On The Cytoplasmic Side Of The Golgi
As Well As On The Vesicles/Buds Originating From It
Alternative Antibody Names Anti-Coatomer Subunit Delta antibody
Anti-Archain antibody
Anti-Delta-Coat Protein antibody
Anti-Delta-Cop antibody
Anti-ARCN1 antibody
Anti-COPD antibody

Information sourced from Uniprot.org

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