• STJ71062 (1µg/ml) staining of A431 (A) and (0. 3ug/ml) of HeLa (B) and MCF7 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71062 (1µg/ml) staining of NIH3T3 (A) , KNRK (B) and MDCK (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71062 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-ARF1 antibody (C-Term) (STJ71062)

SKU:
STJ71062-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/FC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ARF1 (C-Term) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ARF1
Gene ID: 375
Uniprot ID: ARF1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1
Specificity: This antibody is expected to recognize all four reported isoforms.
Immunogen Sequence: EGLDWLSNQLRNQK
Function Small GTPase involved in protein trafficking between different compartments. Modulates vesicle budding and uncoating within the Golgi complex. In its GTP-bound form, triggers the recruitment of coatomer proteins to the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity.the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasticity of excitatory synapses and spine shrinkage during long-term depression (LTD). (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase.
Protein Name Adp-Ribosylation Factor 1
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-1660514
Reactome: R-HSA-167590
Reactome: R-HSA-199992
Reactome: R-HSA-2132295
Reactome: R-HSA-432720
Reactome: R-HSA-432722
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-6811438
Reactome: R-HSA-8950505
Cellular Localisation Golgi Apparatus Membrane
Lipid-Anchor
Cytoplasmic Side
Synapse
Synaptosome
Postsynaptic Density
In The Gdp-Bound Form
Associates Transiently With The Membranes Via Its Myristoylated N-Terminus Where Guanine Nucleotide-Exchange Factor (Gef)-Mediated Nucleotide Exchange Occurs
Following Nucleotide Exchange
The Gtp-Bound Form Undergoes A Conformational Change
Leading To The Exposure Of A Myristoylated N-Terminal Amphipathic Helix That Provides Stable Membrane Anchorage
Alternative Antibody Names Anti-Adp-Ribosylation Factor 1 antibody
Anti-ARF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance