Western Blot Antibodies | St John's Laboratory

Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASCC1 antibody (289-339 aa) (STJ194445)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates using ASCC2 Rabbit polyclonal antibody (STJ115734) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ASCC2 antibody (488-757) (STJ115734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-ASCC2 antibody (STJA0008414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various lysates, using ASCC3 Rabbit pAb (STJ110267) at 1:800 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 50s.

Anti-ASCC3 antibody (1-111) (STJ110267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASCC3 antibody (2053-2103 aa) (STJ195324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of ASCL1 antibody in paraffin-embedded human brain tissue.

Anti-ASCL1 antibody (109-158 aa) (STJ91732)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using AscL1 Rabbit polyclonal antibody (STJ118012) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AscL1 antibody (150-236) (STJ118012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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STJ73536 (1µg/ml) staining of Mouse (A) and Rat (B) Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-ASCL1 antibody (79-91) (STJ73536)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Cow/Mouse/Rat/Dog

€390.63

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ASCL2 antibody (1-80 aa) (STJ94020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Ascl2 antibody (185-263 aa) (STJ11106594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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STJ72471 (0. 1µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Ascl3-Mouse antibody (Internal) (STJ72471)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Cow

€390.63

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Immunohistochemistry analysis of paraffin-embedded human breast cancer using ASCL4 antibody (STJ116649) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ASCL4 antibody (1-70) (STJ116649)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-ASCL4 antibody (81-131) (STJA0006373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Asense antibody at 1:1,000 dilution; protein at 40 ng per lane; chicken polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-Asense antibody (254 aa C-Term) (STJ140181)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

D.melanogaster

€169.38 - €493.13

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Anti-ASF1A antibody (100-204) (STJ116719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Western blot analysis of various cells using Asf1a Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ASF1A antibody (121-170 aa) (STJ91733)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot with Anti-ASF1A Antibody (STJ500171) of rat muscle membranes at 1:500 dilution in buffer.

Anti-ASF1A antibody (60-120) (STJ500171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-ASF1A antibody (60-120) {Biotin} (STJ500172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-ASF1A antibody (60-120) {FITC} (STJ500173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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STJ71364 (1µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASF1A antibody (C-Term) (STJ71364)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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STJ71180 (0. 01µg/ml) staining of Jurkat cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ASF1A/HSPC146 antibody (Internal) (STJ71180)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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Western blot analysis of lysates from K562 cells, using ASF1B Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ASF1B antibody (101-150 aa) (STJ91734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of extracts of various cell lines, using ASF1B antibody (STJ28594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ASF1B antibody (63-202) (STJ28594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-ASGPR antibody [8D7] (STJ16100783)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/ELISA/IF/WB

Reactivity :

Rat/Human

€1,003.13

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Anti-ASGPR/CD301 antibody [121A5.01] (STJ16101996)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/FACS/WB

Reactivity :

Human

€795.63

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Anti-ASGPR/CD301 antibody [121A5.01] {Biotin} (STJ16101995)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

€890.63

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Anti-ASGPR/CD301 antibody [125A10.03] (STJ16101991)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/FACS/WB

Reactivity :

Human/Dog

€795.63

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Anti-ASGPR/CD301 antibody [125A10.03] {Biotin} (STJ16101990)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Dog

€890.63

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using ASGR1 antibody (STJ28951) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ASGR1 antibody (1-150) (STJ28951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of paraffin-embedded mouse liver using ASGR1 Rabbit polyclonal antibody (STJ115244) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ASGR1 antibody (62-609) (STJ115244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-ASGR1 antibody (STJA0006900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various lysates using ASGR2 Rabbit polyclonal antibody (STJ115884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ASGR2 antibody (131-287) (STJ115884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Immunofluorescence analysis of paraffin-embedded Mouse liver tissue using ASGR2 Rabbit polyclonal antibody (STJ28203) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-ASGR2 antibody (58-287) (STJ28203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Anti-ASGR2 antibody (STJA0008909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western Blot analysis of A431 and mouse brain lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-ASH1L antibody (1323-1373 aa) (STJ197834)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-ASH1L antibody (2074-2293 aa) (STJ11106636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Confocal imaging of paraffin-embedded Mouse colon tissue using ASH2L Rabbit monoclonal antibody (STJ11102184, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-ASH2L antibody (529-628) [S4MR] (STJ11102184)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Western blot analysis of various lysates using ASH2L Rabbit pAb (STJ116754) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ASH2L antibody (81-280) (STJ116754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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Anti-ASH2L antibody (STJA0007525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Anti-ASIC1 antibody (100-150 aa) (STJ13100008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human

€414.38

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Anti-ASIC1 antibody (100-150 aa) (STJ13100009)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human

€414.38

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Western blot analysis of mouse-brain, mouse-spinal-cord lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-ASIC1 antibody (220-280 aa) (STJ98706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IHC-F/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of 1) 293T, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with ASIC1 Rabbit pAb diluted at 1:2,000

Anti-ASIC1 antibody (410-490 aa) (STJ197440)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IHC-F

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-ASIC1 antibody (460-505 aa) (STJ13100040)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

€414.38

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Anti-ASIC1 antibody (70-360 aa) (STJ11105986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€140.63 - €655.63

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STJ73108 (0. 3µg/ml) staining of Mouse fetal Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ASIC1 antibody (C-Term) (STJ73108)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

€390.63

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Anti-ASIC1 antibody (STJ13100261)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Human

€414.38

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Anti-ASIC2 antibody (105-155 aa) (STJ13100600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

€414.38

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