Western Blot Antibodies | St John's Laboratory

STJ72737 (0. 3µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ARPC1A antibody (C-Term) (STJ72737)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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Anti-ARPC1B antibody (10-80 aa) (STJ11106282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARPC1B antibody (27-77 aa) (STJ195725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ARPC1B antibody (C-Term) (STJA0005269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Immunohistochemistry analysis of ARPC2 in paraffin-embedded rat spleen using ARPC2 Rabbit polyclonal antibody (STJ112687) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ARPC2 antibody (1-300) (STJ112687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ARPC2 antibody (147-197) (STJA0006392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 300 Mu g extracts from HepG2 cells using 3 Mu g ARPC2 antibody (STJ11102755). Western blot was performed from the immunoprecipitate using ARPC2 antibody (STJ11102755) at a dilution of 1:1000.

Anti-ARPC2 antibody (200-300) [S5MR] (STJ11102755)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunocytochemistry analysis of ARPC2 (green) in Hela using ARPC2 antibody, and DAPI (blue)

Anti-ARPC2 antibody (282-300 aa) [R04-1B6] (STJA0010984)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£246.50

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STJ70177 (0. 5µg/ml) staining of CACO-2 (A) , (0. 1ug/ml) HeLa (B) and Jurkat (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ARPC2 antibody (C-Term) (STJ70177)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Immunofluorescence analysis of A549 cells using ARPC3 antibody (STJ110078). Blue: DAPI for nuclear staining.

Anti-ARPC3 antibody (1-178) (STJ110078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ70540 staining (1µg/ml) of Mouse Brain lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.

Anti-ARPC3 antibody (C-Term) (STJ70540)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Anti-ARPC3 antibody (STJA0007383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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STJ71411 (0. 03µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ARPC4 antibody (Internal) (STJ71411)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse

£312.50

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Anti-ARPC4 antibody (STJA0008256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using p16-ARC/ARC16/ARPC5 Rabbit monoclonal antibody (STJ11102156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-ARPC5 antibody (1-100) [S6MR] (STJ11102156)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using p16-ARC/ARC16/ARPC5 Rabbit pAb (STJ111306) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ARPC5 antibody (1-154) (STJ111306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using p16 ARC antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-ARPC5 antibody (2-20 aa) [R08-2B3] (STJA0010829)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£246.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARPC5 antibody (58-108 aa) (STJ194358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of ARPC5L in paraffin-embedded Human lung cancer using ARPC5L Rabbit polyclonal antibody (STJ118349) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ARPC5L antibody (1-100) (STJ118349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARPC5L antibody (4-54 aa) (STJ194345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARPIN antibody (172-222 aa) (STJ194322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ARPP19 antibody (1-50 aa) (STJ190647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HeLa cells using ARPP-21 Polyclonal Antibody diluted at 1：1000

Anti-ARPP21 antibody (211-260 aa) (STJ91702)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using ARR3 Rabbit polyclonal antibody (STJ114976) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ARR3 antibody (1-260) (STJ114976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from LOVO cells, using ARRC Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ARR3 antibody (339-388 aa) (STJ91707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Beta-arrestin1 Rabbit monoclonal antibody (STJ112640) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ARRB1 antibody (1-100) [S0MR] (STJ112640)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunoprecipitation analysis of 200 Mu g extracts of SH-SY5Y cells using 3 Mu g Beta-arrestin1 antibody (STJ22692). Western blot was performed from the immunoprecipitate using Beta-arrestin1 (STJ22692) at a dilution of 1:1000.

Anti-ARRB1 antibody (169-418) (STJ22692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using Beta-arrestin1 antibody (STJ114874) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ARRB1 antibody (300-418) (STJ114874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Arrestin-Beta-1 Polyclonal Antibody diluted at 1：500

Anti-ARRB1 antibody (369-418 aa) (STJ91708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat and HUVEC cells, using ARRB1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ARRB1 antibody (369-418 aa) (STJ91709)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Arrestin-Beta-1 monoclonal antibody against PC12, Mouse brain, Raw264.7 cell lysate.

Anti-ARRB1 antibody (STJ98467)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot detection of beta Arrestin 1 in PC12, Raw264.7, MCF7 and mouse brain cell lysates using beta Arrestin 1 mouse mAb (1:500 diluted).Predicted band size:50KDa.Observed band size:50KDa.

Anti-ARRB1 antibody [1H4-F5-C11] (STJ99128)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse/Rat/Human

£144.00 - £348.00

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Western blot detection of Beta-Arrestin 1 in K562 and Hela cell lysates using Beta-Arrestin 1 mouse mAb (dilution 1:500).Predicted band size:51kDa.Observed band size:51kDa.

Anti-ARRB1 antibody [6A1-D7-C9] (STJ99310)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£144.00 - £348.00

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Anti-ARRB2 antibody (165-394) (STJ115243)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of NIH/3T3, using ARRB2 antibody (STJ11102772) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ARRB2 antibody (300-400) (STJ11102772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of HeLa cells, using ARRB2 antibody (STJ22694) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ARRB2 antibody (300-400) (STJ22694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Arrestin-Beta-2 monoclonal antibody against HepG2 cell lysate.

Anti-ARRB2 antibody (C-Term) (STJ98468)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Pig

£144.00 - £348.00

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Western blot analysis of various cells using Arrdc1 Polyclonal Antibody diluted at 1：1000

Anti-ARRDC1 antibody (167-216 aa) (STJ91703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ARRDC1-AS1 antibody (80-130 aa) (STJ198082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using ARRDC2 Polyclonal Antibody

Anti-ARRDC2 antibody (141-190 aa) (STJ91704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from rat liver, using ARRDC3 Rabbit pAb (STJ114038) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-ARRDC3 antibody (1-270) (STJ114038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Western blot analysis of lysates from 293T cells, using ARRDC3 Rabbit pAb (STJ114152) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ARRDC3 antibody (1-270) (STJ114152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from rat brain and mouse brain cells, using ARRDC3 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ARRDC3 antibody (365-414 aa) (STJ91705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from COLO cells, using ARRD4 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ARRDC4 antibody (127-176 aa) (STJ91706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from PC-3 cells, using ARRDC4 polyclonal antibody (STJ11100473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ARRDC4 antibody (300-400) (STJ11100473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-ARRDC4 antibody (STJ13100042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£331.50

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STJ70699 (1µg/ml) staining of A549 (A) , and U251 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Arrestin beta 2 antibody (Internal) (STJ70699)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat

£312.50

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Biotinylated STJ70699 (1µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Arrestin beta 2, Biotinylated antibody (Internal) {Biotin} (STJ73227)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat

£312.50

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