Western Blot Antibodies | St John's Laboratory

Immunofluorescence analysis of SH-SY5Y cells using APP Rabbit polyclonal antibody (STJ11103984) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-APP antibody (671-770) (STJ11103984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts from HeLa cells using 3 Mu g [KO Validated] APP Rabbit monoclonal antibody (STJ11101455). Western blot was performed from the immunoprecipitate using [KO Validated] APP Rabbit monoclonal antibody (STJ11101455) at a dilution of 1:1000.

Anti-APP antibody (671-770) [S5MR] (STJ11101455)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, Amyloid-Beta Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-APP antibody (711-760 aa) (STJ91591)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunocytochemistry analysis of Amyloid Precursor Protein (green) in Hela using Amyloid Precursor Protein antibody, and DAPI (blue).

Anti-APP antibody (731-737 aa) [R05-3E3] (STJA0010775)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~115 kDa APP protein.

Anti-APP antibody (C-Term) (STJA0003578)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Canine/Chicken/Human/Non-Human Primates

£454.50

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Immunohistochemical analysis of paraffin-embedded human brain tissue. 1, Polyclonal Antibody was diluted at 1:200 (4°C overnight). 2, EDTA pH 9.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min) Negtive control was used by secondary antibody only.)

Anti-APP antibody (STJ193176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Hamster

£48.00 - £252.00

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Western blot analysis using APP monoclonal antibody against truncated APP recombinant protein.

Anti-APP antibody [1B11F3] (STJ97844)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of various lysates using APPBP2 Rabbit polyclonal antibody (STJ118226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 35s.

Anti-APPBP2 antibody (386-585) (STJ118226)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293 using APP-BP2 antibody.. Secondary antibody was diluted at 1:20000

Anti-APPBP2 antibody (390-470 aa) (STJ97627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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STJ70418 (0. 3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-APPL/DIP13alpha antibody (C-Term) (STJ70418)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of extracts of various cell lines, using APPL1 antibody (STJ22658) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APPL1 antibody (1-210) (STJ22658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using APPL1 Polyclonal Antibody

Anti-APPL1 antibody (121-170 aa) (STJ91648)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using APPL11 Rabbit mAb (STJ11102397) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-APPL1 antibody (350-450) [S7MR] (STJ11102397)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ71081 (0. 5µg/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after infection with Human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.

Anti-APPL1 antibody (Internal) (STJ71081)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of various lysates using APPL2 Rabbit polyclonal antibody (STJ116800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APPL2 antibody (1-300) (STJ116800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-APPL2 antibody (STJA0009099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR3 Rabbit polyclonal antibody (STJ11104778) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-APRR3 antibody (245-371 aa) (STJ11104778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

£146.50 - £642.50

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Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR7 Rabbit polyclonal antibody (STJ11104779) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-APRR7 antibody (236-339 aa) (STJ11104779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

A.thaliana

£146.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using APRT Rabbit polyclonal antibody (STJ115881) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-APRT antibody (1-180) (STJ115881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293T, using CALCA Rabbit polyclonal antibody (STJ27409) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-APRT antibody (1-180) (STJ27409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-APRT antibody (STJA0008078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-APTX antibody (11-60 aa) (STJ192599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunoprecipitation analysis of 150 Mu g extracts of A549 cells using 3 Mu g APTX antibody (STJ27317). Western blot was performed from the immunoprecipitate using APTX antibody (STJ27317) at a dilution of 1:500.

Anti-APTX antibody (93-342) (STJ27317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-AQP0 antibody (210-263 aa) (STJ13100633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat/Guinea Pig

£331.50

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Anti-AQP0 antibody (STJ13100019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Marmoset.

£331.50

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Western blot analysis of lysates from rat brain cells, using AQP1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-AQP1 antibody (101-150 aa) (STJ91652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Confocal imaging of paraffin-embedded Mouse kidney tissue using Aquaporin-1 (AQP1) Rabbit monoclonal antibody (STJ11102015, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to IF staining. Objective: 40x.

Anti-AQP1 antibody (170-269) [S5MR] (STJ11102015)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of human kidney using Aquaporin-1 (AQP1) Rabbit polyclonal antibody (STJ117224) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-AQP1 antibody (200-269) (STJ117224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry of rat kidney with Anti-AQP1 primary antibody (STJ500114) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-AQP1 antibody (215-265) (STJ500114)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Bovine/Human/Mouse/Rat/Sheep

£427.50

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Anti-AQP1 antibody (215-265) {Biotin} (STJ500115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Bovine/Human/Mouse/Rat/Sheep

£528.50

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Immunohistochemistry of rat kidney with Anti-AQP1 primary antibody (FITC) (STJ500116) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-AQP1 antibody (215-265) {FITC} (STJ500116)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Bovine/Human/Mouse/Rat/Sheep

£528.50

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Anti-AQP1 antibody (230-269 aa) (STJ13100013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Aquaporin 1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-AQP1 antibody (255-269 aa) [R03-7G5] (STJA0010975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Rat

£246.50

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Western blot analysis of extracts of various cell lines, using AQP10 antibody (STJ22659) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-AQP10 antibody (209-301) (STJ22659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AQP10 antibody (230-310 aa) (STJ190558)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry of rat kidney with Anti-Aquaporin 10 primary antibody (STJ500117) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-AQP10 antibody (250-300) (STJ500117)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-AQP10 antibody (250-300) {Biotin} (STJ500118)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-AQP10 antibody (250-300) {FITC} (STJ500119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-AQP11 antibody (130-210 aa) (STJ91653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of rat kidney with Anti-Aquaporin 11 primary antibody (STJ500120) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-AQP11 antibody (140-190) (STJ500120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£466.50

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Anti-AQP11 antibody (140-190) {Biotin} (STJ500121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Pig/Rat

£528.50

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Anti-AQP11 antibody (140-190) {FITC} (STJ500122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Pig/Rat

£528.50

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Anti-AQP11 antibody (Internal Region) (STJ13100028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse

£331.50

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Anti-AQP11 antibody (Internal Region) (STJ13100613)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Western blot analysis of various cells using AQP12 Polyclonal Antibody diluted at 1：1000

Anti-AQP12 antibody (231-280 aa) (STJ91654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry with Anti-Aquaporin 12 Antibody (STJ500123) at 1:100 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000B. FFPE section.

Anti-AQP12 antibody (240-290) (STJ500123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

£466.50

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Anti-AQP12 antibody (240-290) {Biotin} (STJ500124)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

£528.50

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Immunohistochemistry of mouse kidney with Anti-Aquaporin 12 primary antibody (FITC) (STJ500125) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-AQP12 antibody (240-290) {FITC} (STJ500125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

£528.50

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