Anti-Annexin 2 antibody [R08-4H4] (STJA0035116)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein of human Annexin-2/ANXA2
General Information
| Short Description | Rabbit monoclonal anti-Annexin 2 for use in WB and IP in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R08-4H4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IP 1:20 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ANXA2 |
| Gene ID | 302 |
| Uniprot ID | ANXA2_HUMAN |
| Immunogen | Recombinant protein of human Annexin-2/ANXA2 |
Additional Info
| Post Translational Modifications | Phosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface. ISGylated. |
| Function | Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. Binds to endosomes damaged by phagocytosis of particulate wear debris and participates in endosomal membrane stabilization, thereby limiting NLRP3 inflammasome activation. Required for endothelial cell surface plasmin generation and may support fibrinolytic surveillance and neoangiogenesis. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen. |
| Protein Name | Annexin A2Annexin IiAnnexin-2Calpactin I Heavy ChainCalpactin-1 Heavy ChainChromobindin-8Lipocortin IiPlacental Anticoagulant Protein IvPap-IvProtein IP36 |
| Database Links | Reactome: R-HSA-445355Reactome: R-HSA-6798695Reactome: R-HSA-75205Reactome: R-HSA-8950505Reactome: R-HSA-9860927 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneMelanosomeIn The Lamina Beneath The Plasma MembraneIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvTranslocated From The Cytoplasm To The Cell Surface Through A Golgi-Independent Mechanism |
| Alternative Antibody Names | Anti-Annexin A2 antibodyAnti-Annexin Ii antibodyAnti-Annexin-2 antibodyAnti-Calpactin I Heavy Chain antibodyAnti-Calpactin-1 Heavy Chain antibodyAnti-Chromobindin-8 antibodyAnti-Lipocortin Ii antibodyAnti-Placental Anticoagulant Protein Iv antibodyAnti-Pap-Iv antibodyAnti-Protein I antibodyAnti-P36 antibodyAnti-ANXA2 antibodyAnti-ANX2 antibodyAnti-ANX2L4 antibodyAnti-CAL1H antibodyAnti-LPC2D antibody |
Information sourced from Uniprot.org