| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ANLN (845-1124) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ANLN |
| Gene ID: |
54443 |
| Uniprot ID: |
ANLN_HUMAN |
| Immunogen Region: |
845-1124 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 845-1124 of human ANLN (NP_061155.2). |
| Immunogen Sequence: |
ATPLASTSNSLNGDALTFTT TFTLQDVSNDFEINIEVYSL VQKKDPSGLDKKKKTSKSKA ITPKRLLTSITTKSNIHSSV MASPGGLSAVRTSNFALVGS YTLSLSSVGNTKFVLDKVPF LSSLEGHIYLKIKCQVNSSV EERGFLTIFEDVSGFGAWHR RWCVLSGNCISYWTYPDDEK RKNPIGRINLANCTSRQIEP ANREFCARRNTFELITVRPQ REDDRETLVSQCRDTLCVT |
| Tissue Specificity | Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus. Expression is significantly increased in renal biopsy specimens from idiopathic FSGS. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors. |
| Post Translational Modifications | Phosphorylated during mitosis. Ubiquitinated, and this requires FZR1/CDH1. |
| Function | Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis. May play a significant role in podocyte cell migration. |
| Protein Name | Anillin |
| Database Links | Reactome: R-HSA-8980692Reactome: R-HSA-9013026Reactome: R-HSA-9013106 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonCell CortexCell ProjectionBlebMainly Found In The Nucleus During InterphaseColocalizes With Cortical F-Actin Upon Nuclear Envelope Breakdown In Mitosis And Subsequently Concentrates In The Area Of The Prospective Contractile Ring In AnaphaseThis Pattern Persists Until TelophaseWhen The Protein Becomes Concentrated In The Midbody |
| Alternative Antibody Names | Anti-Anillin antibodyAnti-ANLN antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
