Anti-Annexin A2 antibody (N-Term) (STJ70118)

SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70118-100
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General Information

Short DescriptionGoat polyclonal antibody anti-Annexin A2 (N-Term) is suitable for use in ELISA and Western Blot research applications.
ApplicationsPep-ELISA/WB
HostGoat
ReactivityHuman/Mouse/Rat/Cow/Pig
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:1000.
WB: Approx 35kDa band observed in lysates of cell lines A549, HeLa and NIH3T3 (calculated MW of 38.6kDa according to NP_004030.1 and NP_001002858.1). Recommended concentration: 0.5-3µg/ml.
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20°C on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolANXA2
Gene ID302
Uniprot IDANXA2_HUMAN
Accession NumberNP_001002858.1; NP_004030.1
Immunogen RegionN-Term
Immunogen SequenceSTVHEILCKLSLEGD
SpecificityThis antibody is expected to recognise both reported isoforms (NP_001002858.1; NP_004030.1). Variants NP_004030.1, NP_001002857.1 and NP_001129487.1 represent identical protein.

Additional Info

Function Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. Inhibits PCSK9-enhanced LDLR degradation, probably reduces PCSK9 protein levels via a translational mechanism but also competes with LDLR for binding with PCSK9. (Microbial infection) Binds M.pneumoniae CARDS toxin, probably serves as one receptor for this pathogen. When ANXA2 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen.
Protein Name Annexin A2
Annexin Ii
Annexin-2
Calpactin I Heavy Chain
Calpactin-1 Heavy Chain
Chromobindin-8
Lipocortin Ii
Placental Anticoagulant Protein Iv
Pap-Iv
Protein I
P36
Database Links Reactome: R-HSA-445355
Reactome: R-HSA-6798695
Reactome: R-HSA-75205
Reactome: R-HSA-8950505
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
Melanosome
In The Lamina Beneath The Plasma Membrane
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Translocated From The Cytoplasm To The Cell Surface Through A Golgi-Independent Mechanism
Alternative Antibody Names Anti-Annexin A2 antibody
Anti-Annexin Ii antibody
Anti-Annexin-2 antibody
Anti-Calpactin I Heavy Chain antibody
Anti-Calpactin-1 Heavy Chain antibody
Anti-Chromobindin-8 antibody
Anti-Lipocortin Ii antibody
Anti-Placental Anticoagulant Protein Iv antibody
Anti-Pap-Iv antibody
Anti-Protein I antibody
Anti-P36 antibody
Anti-ANXA2 antibody
Anti-ANX2 antibody
Anti-ANX2L4 antibody
Anti-CAL1H antibody
Anti-LPC2D antibody

Information sourced from Uniprot.org

Citations

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