Western Blot Antibodies | St John's Laboratory

Western blot analysis of K562, L929 cells using CD84 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD84 antibody (131-180 aa) (STJ96621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using CD84 antibody (STJ28516). Blue: DAPI for nuclear staining.

Anti-CD84 antibody (22-225) (STJ28516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CD84 antibody (STJ11103482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD84 antibody (246-345) [S2MR] (STJ11103482)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-CD84 antibody [153-4D9] (STJ16100008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Western blot analysis of extracts of various cell lines, using CD86 antibody (STJ11101564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CD86 antibody (1-100) [S4MR] (STJ11101564)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using CD86 Rabbit polyclonal antibody (STJ11105173) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD86 antibody (24-245 aa) (STJ11105173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of CD86 in paraffin-embedded mouse spleen using CD86 Rabbit polyclonal antibody (STJ113561) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD86 antibody (24-247) (STJ113561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunofluorescence analysis of Raji cells using CD86 Rabbit polyclonal antibody (STJ23014) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD86 antibody (25-133) (STJ23014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells using 3 Mu g CD86 antibody (STJ119185). Western blot was performed from the immunoprecipitate using CD86 antibody (STJ119185) at a dilution of 1:1000.

Anti-CD86 antibody (25-329) (STJ119185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-CD86 Antibody (STJ500486) with peptide on at 1:500 dilution in buffer.

Anti-CD86 antibody (250-329) (STJ500486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£427.50

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Anti-CD86 antibody (250-329) {Biotin} (STJ500487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Anti-CD86 antibody (250-329) {FITC} (STJ500488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Anti-CD86 antibody (STJA0010129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using CD86 monoclonal antibody against L1210 (1) and MOLT-4 (2) cell lysate.

Anti-CD86 antibody [1B3] (STJ97939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-CD86 antibody [BU63] (STJA0001270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/Block/IP

Reactivity :

Human

£123.50 - £187.50

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Anti-CD88 antibody [P12/1] (STJ16100547)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC/IHC-F

Reactivity :

Human/Rhesus

£938.50

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Anti-CD88 antibody [S5/1] (STJ16100838)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/FC

Reactivity :

Human/Rabbit/Ferret/Bovine/Mustelid/Mink

£938.50

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD8 a (ABT155) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MOLT-4Predicted band size: 26kDaObserved band size: 35kDa

Anti-CD8A antibody (100-235 aa) [ABT155] (STJ197126)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-CD8 a (ABT455) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Daudi Lane 2: Raji Lane 3: MOLT-4Predicted band size: 26kDaObserved band size: 24kDa

Anti-CD8A antibody (100-235 aa) [ABT304] (STJ197218)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of extracts of various cell lines, using CD8A rabbit monoclonal antibody (STJ11101251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-CD8A antibody (136-235) [S1MR] (STJ11101251)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-CD8A antibody (22-182 aa) [SRM] (STJ11106940)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil using CD8A Rabbit polyclonal antibody (STJ114922) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD8A antibody (22-235) (STJ114922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates, using CD8A Rabbit polyclonal antibody (STJ11104719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CD8A antibody (STJ11104719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-CD8A antibody (STJA0010229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Cd8a antibody [53-6.7] (STJA0001670)

Clonality :

Monoclonal

Host :

Rat

Applications :

Block/Depletion/FC/WB/IF/IP

Reactivity :

Mouse

£93.50 - £188.50

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Anti-Cd8a antibody [53-6.7] (STJA0001671)

Clonality :

Monoclonal

Host :

Rat

Applications :

FC/WB/IF/IP

Reactivity :

Mouse

£93.50 - £114.50

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD8B using CD8B Rabbit polyclonal antibody (STJ23016) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD8B antibody (22-170) (STJ23016)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CD8B antibody (STJ192945)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using CD9 Polyclonal Antibody diluted at 1：1000

Anti-CD9 antibody (101-150 aa) (STJ92147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of CD9 in paraffin-embedded human smooth muscle using CD9 Rabbit polyclonal antibody (STJ23017) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD9 antibody (112-195) (STJ23017)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CD9 antibody (115-195 aa) (STJ140294)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Reacts against human, rat, mouse, canine and monkey proteins.

£135.50 - £394.50

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Immunoprecipitation analysis of 300 Mu g extracts from THP-1 cells using 3 Mu g CD9 Rabbit monoclonal antibody (STJ11101565). Western blot was performed from the immunoprecipitate using CD9 Rabbit monoclonal antibody (STJ11101565) at a dilution of 1:1000.

Anti-CD9 antibody (129-228) [S5MR] (STJ11101565)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Anti-CD9 antibody (N-Term) (STJA0005794)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£455.50

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Anti-CD9 antibody [BU16] (STJ16101389)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/FC/IF

Reactivity :

Human

£741.50

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Anti-CD9 antibody [MM2/57] (STJ16100822)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/IHC-F

Reactivity :

Human/Llama/Mink/Mustelid/Ferret/Dog/Horse/Bovine/Cat/Rhesus/Pig/Rabbit

£741.50

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Western blot analysis of Mouse spleen, using CD90. 1 Rabbit polyclonal antibody (STJ11105183) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD90.1 antibody (20-120 aa) (STJ11105183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human liver using CD90/Thy1 Rabbit monoclonal antibody (STJ11102747) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD90/Thy1 antibody (1-100) [S7MR] (STJ11102747)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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STJ71954 (0. 1µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD90/Thy1 antibody (Internal) (STJ71954)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Dog/Pig

£312.50

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Anti-CD91 antibody [A2Mr alpha-2] (STJ16100538)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/FC/IHC-F/ELISA

Reactivity :

Human

£646.50

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Western blot analysis of KB cells using CD93 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD93 antibody (191-240 aa) (STJ97292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CD96 Rabbit polyclonal antibody (STJ11103987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CD96 antibody (270-370) (STJ11103987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293-UV cells using CD96 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-CD96 antibody (291-340 aa) (STJ97293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of Mouse lung, using CD97 Rabbit polyclonal antibody (STJ11105186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD97 antibody (24-265 aa) (STJ11105186)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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STJ72921 (0. 3µg/ml) staining of Mouse Thymus lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Cd97-Mouse antibody (C-Term) (STJ72921)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Mouse

£312.50

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Western blot analysis of various lysates, using CD98 antibody (STJ11104992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD98 antibody (105-526 aa) (STJ11104992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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LN18 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD99 (ABT156) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: LN18Predicted band size: 19kDaObserved band size: 28kDa

Anti-CD99 antibody (1-100 aa) [ABT156] (STJ197127)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of various lysates using CD99 Rabbit mAb (STJ11105761) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-CD99 antibody (1-100) [S5761RM] (STJ11105761)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various lysates, using CD99 Rabbit polyclonal antibody (STJ11105426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) :CHOExposure time: 90s.

Anti-CD99 antibody (23-124) (STJ11105426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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