Western Blot Antibodies | St John's Laboratory

Biotinylated STJ72089 (0. 1µg/ml) staining of Mouse (A) and Rat (B) Liver lysates (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ACAT1, Biotinylated antibody (253-266) {Biotin} (STJ73206)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Cow

$421.88

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Biotinylated STJ72088 (0. 1µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ACAT1, Biotinylated antibody (257-269) {Biotin} (STJ73217)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Cow

$421.88

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Western blot analysis of extracts of various cell lines, using ACAT2 antibody (STJ11102759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ACAT2 antibody (150-250) [S9MR] (STJ11102759)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various lysates using ACAT2 Rabbit pAb (STJ110176) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ACAT2 antibody (159-277) (STJ110176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ACAT2 antibody (320-370 aa) (STJ194911)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of L929 cells using ACBD3 Rabbit polyclonal antibody (STJ118995) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ACBD3 antibody (1-160) (STJ118995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ACBD3 antibody (STJA0009630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ACBD4 antibody (110-190 aa) (STJ190502)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using ACBD5 antibody (STJ112608) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACBD5 antibody (170-440) (STJ112608)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-ACBD5 antibody (250-330 aa) (STJ190503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various cells using ACBD6 Polyclonal Antibody diluted at 1：1000

Anti-ACBD6 antibody (121-170 aa) (STJ91437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunoprecipitation analysis of 300 Mu g extracts of HepG2 cells using 3 Mu g ACC1 antibody (STJ11103059). Western blot was performed from the immunoprecipitate using ACC1 (STJ11103059) at a dilution of 1:1000.

Anti-ACC1 antibody (350-450) [S9MR] (STJ11103059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-accD antibody (STJ11106773)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

M.esculenta

$197.78 - $867.38

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Anti-ACCN2 antibody (ECD) (STJ13100575)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human

$447.53

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Anti-ACCN4 antibody (10-60 aa) (STJ13100603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

$447.53

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Anti-ACCN4 antibody (480-530 aa) (STJ13100602)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

$447.53

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Western blot analysis of lysates from 293T cells, using ACCS Rabbit pAb (STJ115402) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ACCS antibody (222-501) (STJ115402)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of various lysates using ACCS Rabbit pAb (STJ29532) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ACCS antibody (222-501) (STJ29532)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ACCS antibody (242-292) (STJA0006546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ACCSL antibody (453-503 aa) (STJ193612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from wild type (WT) and ACD knockdown (KD) 293T (KD) cells, using ACD Rabbit polyclonal antibody (STJ114070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ACD antibody (1-220) (STJ114070)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-ACD antibody (STJA0008363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse kidney cells using 3 Mu g ACE1 antibody (STJ11101316). Western blot was performed from the immunoprecipitate using ACE1 antibody (STJ11101316) at a dilution of 1:1000.

Anti-ACE antibody (1200-1306) [S6MR] (STJ11101316)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Mouse/Rat

$174.83 - $775.58

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Immunohistochemistry analysis of paraffin-embedded mouse lung using ACE11 Rabbit polyclonal antibody (STJ22482) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ACE antibody (554-758) (STJ22482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of HuvEc cells using ACE1 Polyclonal Antibody diluted at 1：1000

Anti-ACE antibody (891-940 aa) (STJ91441)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis using ACE1 monoclonal antibody against mouse kidney lysate.

Anti-ACE antibody (N-Term) (STJ98458)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow

$194.40 - $469.80

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Western blot detection of Angiotensin Converting Enzyme 1 in Mouse kidney cell lysates using Angiotensin Converting Enzyme 1 mouse mAb (1:1000 diluted).Predicted band size:150KDa.Observed band size:190KDa.

Anti-ACE antibody [4F3-E6-H8] (STJ99092)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse

$194.40 - $469.80

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Anti-ACE2 antibody (18-740 aa) [SRM] (STJ11106076)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of paraffin-embedded mouse kidney using ACE2 Rabbit polyclonal antibody (STJ114610) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ACE2 antibody (18-740) (STJ114610)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ACE2 antibody (STJ11102116) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ACE2 antibody (18-740) [S6MR] (STJ11102116)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-ACE2 antibody (190-240 aa) (STJ13100524)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Mouse/Rat

$447.53

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Anti-ACE2 antibody (200-250 aa) (STJ13100718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

$447.53

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Western blot analysis of lysate from HeLa cells, using ACE2 antibody.

Anti-ACE2 antibody (416-465 aa) (STJ91442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of 293T transfected ACE2 cell lysate using Rabbit pAb diluted at 1:5,000.

Anti-ACE2 antibody (425-475) (STJA0006717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-ACE2 antibody (750-800 aa) (STJ13100521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

$447.53

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Anti-ACE2 antibody (750-800 aa) (STJ13100523)

Clonality :

Polyclonal

Host :

Guinea Pig

Applications :

IHC/WB

Reactivity :

Mouse/Rat

$447.53

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STJ73761 (0. 5µg/ml) staining of Human Kidney lysate (A) + Peptide (B) (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ACE2 antibody (C-Term) (STJ73761)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

$421.88

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Anti-ACE2 antibody (Internal) (STJ73769)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

$421.88

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STJ73762 (1. 5µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ACE2 antibody (N-Term) (STJ73762)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/FC

Reactivity :

Human

$421.88

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Anti-ACE2 antibody (Ser19-Ser740) [1A429] (STJA0018419)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

$309.83 - $443.48

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Western blot analysis of 1 293T cell, 2 293T Transfected ACE2 CellLysate using ACE2 mouse Monoclonla Antibody diluted at 1:50,000.

Anti-ACE2 antibody [2C1] (STJA0006217)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-ACE2 antibody [SAA0323] (STJA0019235)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/FC/WB

Reactivity :

Human

$461.03 - $1,954.13

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Western blot analysis of HepG2 using ASAH3 antibody.. Secondary antibody was diluted at 1:20000

Anti-ACER1 antibody (100-180 aa) (STJ97625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Western blot analysis of COS7 using ASAH3L antibody.. Secondary antibody was diluted at 1:20000

Anti-ACER2 antibody (50-130 aa) (STJ97626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

$64.80 - $340.20

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Western blot analysis of various cells using APHC Polyclonal Antibody

Anti-ACER3 antibody (171-220 aa) (STJ91628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using ACC1 antibody (STJ118055) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Acetyl-ACACA antibody (2266-2346) (STJ118055)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of 1, 293T 2, hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-Acetyl-ACSS1-K642 antibody (STJ98836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Acetyl-ACSS2-Lys418 antibody (STJA0005918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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