Anti-ACADM antibody (1-220) (STJ29869)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ACADM (1-220) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ACADM |
| Gene ID | 34 |
| Uniprot ID | ACADM_HUMAN |
| Immunogen Region | 1-220 |
| Immunogen Sequence | MAAGFGRCCRVLRSISRFHW RSQHTKANRQREPGLGFSFE FTEQQKEFQATARKFAREEI IPVAAEYDKTGEYPVPLIRR AWELGLMNTHIPENCGGLGL GTFDACLISEELAYGCTGVQ TAIEGNSLGQMPIIIAGNDQ QKKKYLGRMTEEPLMCAYCV TEPGAGSDVAGIKTKAEKKG DEYIINGQKMWITNGGKANW YFLLARSDPDPKAPANKAFT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACADM (NP_000007.1). |
Additional Info
| Post Translational Modifications | Acetylated. Could occur at proximity of the cofactor-binding sites and reduce the catalytic activity. Could be deacetylated by SIRT3. |
| Function | Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains. |
| Protein Name | Medium-Chain Specific Acyl-Coa Dehydrogenase - MitochondrialMcadMedium Chain Acyl-Coa DehydrogenaseMcadh |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-77288Reactome: R-HSA-77346Reactome: R-HSA-77348 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Medium-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibodyAnti-Mcad antibodyAnti-Medium Chain Acyl-Coa Dehydrogenase antibodyAnti-Mcadh antibodyAnti-ACADM antibody |
Information sourced from Uniprot.org