• Biotinylated STJ70259 (3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ACADM, Biotinylated antibody (C-Term) {Biotin} (STJ73260)

SKU:
STJ73260-100

£303.50
Shipping:
Free Shipping
Current Stock:
Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ACADM, Biotinylated (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-2-4µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ACADM
Gene ID: 34
Uniprot ID: ACADM_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000007.1; NP_001120800.1; NP_001272971.1; NP_001272972.1; NP_001272973.1
Specificity: This antibody is expected to recognise both reported isoforms (NP_000007.1; NP_001120800.1).
Immunogen Sequence: RLIVAREHIDKYKN.
Function Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains.
Protein Name Medium-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial
Mcad
Medium Chain Acyl-Coa Dehydrogenase
Mcadh
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-77288
Reactome: R-HSA-77346
Reactome: R-HSA-77348
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Medium-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibody
Anti-Mcad antibody
Anti-Medium Chain Acyl-Coa Dehydrogenase antibody
Anti-Mcadh antibody
Anti-ACADM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance