• Western blot analysis of extracts of various cell lines, using ACAD9 antibody (STJ116056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ACAD9 antibody (1-270) (STJ116056)

SKU:
STJ116056

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACAD9 (1-270) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACAD9
Gene ID: 28976
Uniprot ID: ACAD9_HUMAN
Immunogen Region: 1-270
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACAD9 (NP_054768.2).
Immunogen Sequence: MSGCGLFLRTTAAARACRGL VVSTANRRLLRTSPPVRAFA KELFLGKIKKKEVFPFPEVS QDELNEINQFLGPVEKFFTE EVDSRKIDQEGKIPDETLEK LKSLGLFGLQVPEEYGGLGF SNTMYSRLGEIISMDGSITV TLAAHQAIGLKGIILAGTEE QKAKYLPKLASGEHIAAFCL TEPASGSDAASIRSRATLSE DKKHYILNGSKVWITNGGLA NIFTVFAKTEVVDSDGSVK
Tissue Specificity Ubiquitously expressed in most normal human tissues and cancer cell lines with high level of expression in heart, skeletal muscles, brain, kidney and liver. In the cerebellum uniquely expressed in the granular layer (at protein level).
Function As part of the MCIA complex, primarily participates in the assembly of the mitochondrial complex I and therefore plays a role in oxidative phosphorylation. This moonlighting protein has also a dehydrogenase activity toward a broad range of substrates with greater specificity for long-chain unsaturated acyl-CoAs. However, in vivo, it does not seem to play a primary role in fatty acid oxidation. In addition, the function in complex I assembly is independent of the dehydrogenase activity of the protein.
Protein Name Complex I Assembly Factor Acad9 - Mitochondrial
Acyl-Coa Dehydrogenase Family Member 9
Acad-9
Database Links Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Essentially Associated With Membranes
Alternative Antibody Names Anti-Complex I Assembly Factor Acad9 - Mitochondrial antibody
Anti-Acyl-Coa Dehydrogenase Family Member 9 antibody
Anti-Acad-9 antibody
Anti-ACAD9 antibody

Information sourced from Uniprot.org

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