Immunofluorescence Antibodies | St John's Laboratory

Confocal imaging of U-2 OS cells using GCSH Rabbit mAb (STJ11107342, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (STJS001166, dilution 1:500) (Red). The cells were counterstained with Î±-Tubulin Mouse mAb (STJ11107556, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (STJS001209, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-GCSH antibody (49-173 aa) [SRM] (STJ11107342)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunofluorescence analysis of A549 cells using GDF15 Rabbit polyclonal antibody (STJ11105532) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GDF15 antibody (197-308) (STJ11105532)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of HT-1080 cells using 3 Mu g GDF15 antibody (STJ23764). Western blot was performed from the immunoprecipitate using GDF15 antibody (STJ23764) at a dilution of 1:1000.

Anti-GDF15 antibody (49-308) (STJ23764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200

Anti-GDF2 antibody (371-420 aa) (STJ98776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of 293 cells using GDF-5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GDF5 antibody (361-410 aa) (STJ96667)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Hela cells using GDF-7 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-GDF7 antibody (321-370 aa) (STJ97349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using GDF-9 Polyclonal Antibody

Anti-GDF9 antibody (273-322 aa) (STJ93256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using [KO Validated] GDI2 Rabbit pAb (STJ111343) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-GDI2 antibody (296-445) (STJ111343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of A549, HT29, K562, mouse kidney, mouse lung cells using GDI-2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GDI2 antibody (C-Term) (STJ96682)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using GDNF antibody (STJ116846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GDNF antibody (109-211) (STJ116846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-GDNF antibody [3C1] (STJA0002911)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/IF/IHC

Reactivity :

Human

£438.50 - £2,502.50

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Immunofluorescence analysis of MCF7 cells using GDNF Receptor alpha 1/GFRA1 Rabbit mAb (STJ11107301) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GDNF Receptor alpha 1/GFRA1 antibody (361-460 aa) [SRM] (STJ11107301)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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STJ70329 (4µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-GERP/TRIM8 antibody (C-Term) (STJ70329)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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Western blot analysis of extracts of various cell lines, using GFAP antibody (STJ11101596) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-GFAP antibody (1-100) [S6MR] (STJ11101596)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of mouse brain, using GFAP rabbit polyclonal antibody (STJ23770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-GFAP antibody (1-75) (STJ23770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, GFAP Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-GFAP antibody (11-60 aa) (STJ93259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-GFAP antibody (300-432 aa) [ABT-GFAP] (STJ196765)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-GFAP (ABT176) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Neuro-2aPredicted band size: 50kDaObserved band size: 57kDa

Anti-GFAP antibody (300-432 aa) [ABT176] (STJ197157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Human astrocytoma tissue was stained with Anti-Glial Fibrillary Acidic Protein (GFAP) (ABT331) Antibody

Anti-GFAP antibody (300-432 aa) [ABT331] (STJ197231)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Anti-GFAP antibody (300-432 aa) [ABT484] (STJ197230)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Human cerebrum tissue was stained with Anti-Glial Fibrillary Acidic Protein (GFAP) (ABT518) Antibody

Anti-GFAP antibody (300-432 aa) [PT1995] (STJ196968)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of U-251MG cells using GFAP Rabbit polyclonal antibody (STJ116876) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-GFAP antibody (350-432) (STJ116876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of Hela cell. 1, AR Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-GFAP antibody [5C8] (STJ96961)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of GFAP at dilution of 1:1000

Anti-GFAP antibody [SM0401] (STJA0010401)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-GFAP antibody [SM0405] (STJA0010405)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-GFAP antibody [SM0407] (STJA0010407)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£170.50 - £233.50

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Anti-GFAP antibody [SM5031] (STJA0005031)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of HBE cells using ALR Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GFER antibody (51-100 aa) (STJ96894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-GFM2 antibody (441-490 aa) (STJ92842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-GFP antibody at 1:1000 dilution; HEK293 transfected cell lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-GFP antibody (STJ140005)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

GFP

£86.50 - £534.50

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Anti-GFP antibody at 1:2000 dilution; transfected 293HEK cell lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-GFP antibody (STJ140006)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

GFP

£86.50 - £534.50

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Anti-GFP antibody (STJ140285)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

GFP

£101.50 - £394.50

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Anti-GFP antibody {DyLight®405} (STJ140274)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

GFP

£141.50 - £426.50

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Anti-GFP antibody {DyLight®488} (STJ140275)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

GFP

£141.50 - £426.50

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Immunofluorescence in Drosophila larvae NMJ syt1-GFP (syt1 is a synaptic protein) muscle 6/7 using anti-GFP conjugated to DyLight®550 at 1/500

Anti-GFP antibody {DyLight®550} (STJ140276)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

GFP

£141.50 - £426.50

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Anti-GFP antibody {DyLight®633} (STJ140277)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

GFP

£141.50 - £426.50

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Anti-GFP antibody {DyLight®650} (STJ140313)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF/IHC-F

Reactivity :

Green Fluorescent Protein.

£141.50 - £442.50

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Anti-GFP antibody {DyLight®680} (STJ140314)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF/IHC-F

Reactivity :

Green Fluorescent Protein.

£141.50 - £442.50

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Anti-GFP antibody {DyLight®800} (STJ140315)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF/IHC-F

Reactivity :

Green Fluorescent Protein.

£141.50 - £442.50

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Western blot analysis of extracts of normal 293T cells and 293T transfected with GFP-tagged fusion protein, using Rabbit anti GFP-Tag pAb (STJ11103701) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-GFP-Tag antibody (1-238) (STJ11103701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Species independent

£135.50 - £249.50

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Chromatin immunoprecipitation was performed with cross-linked chromatin from Arabidopsis thaliana leaf cells transfered with GFP, using Rabbit anti GFP-Tag mAb (STJ11103711) and Rabbit IgG (STJ11105763). The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.

Anti-GFP-Tag antibody (1-238) [S1MR] (STJ11103711)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA/ChIP

Reactivity :

Species independent

£135.50 - £249.50

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1ug GFP fusion protein+ primary antibody dilution at 1) 1:50002) 1:20000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-GFP-Tag antibody (STJ197379)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF

Reactivity :

Species independent

£48.00 - £252.00

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Anti-GFP-Tag antibody [1G6] {HRP} (STJA0006065)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Species independent

£144.00 - £240.00

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Western blot analysis using GFP monoclonal antibody against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2).

Anti-GFP-Tag antibody [4B10B2] (STJ98108)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Species independent

£144.00 - £348.00

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Western blot analysis of GFP transfected Hela, diluted at, 1) 1:5000 , 2) 1:10000

Anti-GFP-Tag antibody [Mix] (STJ97026)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP/CoIP

Reactivity :

Species independent

£48.00 - £252.00

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Anti-GFP-Tag antibody {HRP} (STJA0006066)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF

Reactivity :

Species independent

£144.00 - £240.00

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Immunofluorescence analysis of L929 cells using GFPT2 antibody (STJ117569) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-GFPT2 antibody (200-260) (STJ117569)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-GFRa-1 antibody (STJA0002877)

Clonality :

Polyclonal

Host :

Chicken

Applications :

ELISA/WB/IF

Reactivity :

Human

£438.50 - £2,502.50

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