• Western blot analysis of extracts of Rat liver, using CYP2E1 rabbit polyclonal antibody antibody (STJ23323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using CYP2E1 antibody (STJ23323) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using CYP2E1 antibody (STJ23323) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse liver cells using CYP2E1 rabbit polyclonal antibody (STJ23323) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat liver cells using CYP2E1 rabbit polyclonal antibody (STJ23323) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-CYP2E1 antibody (234-493) (STJ23323)

SKU:
STJ23323

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYP2E1 (234-493) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP2E1
Gene ID: 1571
Uniprot ID: CP2E1_HUMAN
Immunogen Region: 234-493
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1).
Immunogen Sequence: KVIKNVAEVKEYVSERVKEH HQSLDPNCPRDLTDCLLVEM EKEKHSAERLYTMDGITVTV ADLFFAGTETTSTTLRYGLL ILMKYPEIEEKLHEEIDRVI GPSRIPAIKDRQEMPYMDAV VHEIQRFITLVPSNLPHEAT RDTIFRGYLIPKGTVVVPTL DSVLYDNQEFPDPEKFKPEH FLNENGKFKYSDYFKPFSTG KRVCAGEGLARMELFLLLCA ILQHFNLKPLVDPKDIDLS
Function A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics (Probable).
Protein Name Cytochrome P450 2e1
4-Nitrophenol 2-Hydroxylase
Cypiie1
Cytochrome P450-J
Database Links Reactome: R-HSA-211981
Reactome: R-HSA-211999
Reactome: R-HSA-9027307
Reactome: R-HSA-9749641
Reactome: R-HSA-9753281
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Microsome Membrane
Mitochondrion Inner Membrane
Post-Translationally Targeted To Mitochondria
Tomm70 Is Required For The Translocation Across The Mitochondrial Outer Membrane
After Translocation Into The Matrix
Associates With The Inner Membrane As A Membrane Extrinsic Protein
Alternative Antibody Names Anti-Cytochrome P450 2e1 antibody
Anti-4-Nitrophenol 2-Hydroxylase antibody
Anti-Cypiie1 antibody
Anti-Cytochrome P450-J antibody
Anti-CYP2E1 antibody
Anti-CYP2E antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance