ELISA Antibodies | St John's Laboratory

Western blot analysis of various lysates using EIF2S2 Rabbit polyclonal antibody (STJ11102835) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EIF2S2 antibody (1-175) (STJ11102835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EIF2S2 antibody (10-90 aa) (STJ92873)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using EIF2S2 Rabbit pAb (STJ28167) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-EIF2S2 antibody (134-333) (STJ28167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of the lysates from HT-29 cells using EIF2S2 antibody.

Anti-EIF2S2 antibody (61-110 aa) (STJ92874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded rat brain using EIF2S3 antibody (STJ28664) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-EIF2S3 antibody (1-250) (STJ28664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of rat brain, using EIF2S3 antibody (STJ23510) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF2S3 antibody (173-472) (STJ23510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$151.88 - $708.08

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Immunofluorescence analysis of U2OS cells using EIF3A antibody (STJ29809) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-EIF3A antibody (1-250) (STJ29809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-EIF3A antibody (STJ190814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of NIH-3T3 cells using eIF3B Polyclonal Antibody (STJ112297) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF3B antibody (100-350) (STJ112297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using eIF3B Rabbit mAb (STJ11102435) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EIF3B antibody (101-200) [S5MR] (STJ11102435)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of U2OS cells using EIF3C antibody (STJ29102). Blue: DAPI for nuclear staining.

Anti-EIF3C antibody (694-913) (STJ29102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-EIF3D antibody (1-240) (STJ27743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EIF3D Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF3D antibody (101-150 aa) (STJ92879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using eIF3e antibody (STJ27400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF3E antibody (1-445) (STJ27400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded mouse testis using eIF3e Rabbit monoclonal antibody (STJ11101868) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-EIF3E antibody (210-350) [S8MR] (STJ11101868)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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STJ72427 (0. 03µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-EIF3E antibody (C-Term) (STJ72427)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Dog/Cow/Pig

$421.88

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Western blot analysis of various cells using eIF3 Epsilon Polyclonal Antibody

Anti-EIF3F antibody (81-130 aa) (STJ92878)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of HeLa, using EIF3F rabbit polyclonal antibody (STJ29103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-EIF3F antibody (88-357) (STJ29103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U-2 OS cells using EIF3G Polyclonal Antibody (STJ23512) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF3G antibody (1-320) (STJ23512)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells using 1 Mu g EIF3H antibody (STJ115340). Western blot was performed from the immunoprecipitate using EIF3H antibody (STJ115340) at a dilution of 1:1000.

Anti-EIF3H antibody (80-340) (STJ115340)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from wild type (WT) and EIF3H knockout (KO) HeLa cells, using [KO Validated] EIF3H Rabbit polyclonal antibody (STJ119999) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF3H antibody (80-340) (STJ119999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of various lysates using EIF3J Rabbit pAb (STJ113577) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-EIF3J antibody (1-258) (STJ113577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-EIF3J antibody (40-120 aa) (STJ92877)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using EIF3K Rabbit pAb (STJ112010) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-EIF3K antibody (1-218) (STJ112010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using eIF3K Polyclonal Antibody diluted at 1：1000

Anti-EIF3K antibody (61-110 aa) (STJ92875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using EIF3L Rabbit pAb (STJ112013) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-EIF3L antibody (1-230) (STJ112013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various cells using eIF3L Polyclonal Antibody diluted at 1：1000

Anti-EIF3L antibody (1-50 aa) (STJ92876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of EIF3M in paraffin-embedded rat brain using EIF3M Rabbit polyclonal antibody (STJ23513) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-EIF3M antibody (1-374) (STJ23513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-EIF4A1 antibody (1-100) (STJ27247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U-2 OS cells using eIF4A Rabbit polyclonal antibody (STJ11103240) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF4A1/EIF4A2/EIF4A3 antibody (1-100) (STJ11103240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using EIF4A2 antibody (STJ29906) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF4A2 antibody (1-270) (STJ29906)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using eIF4A3 Rabbit polyclonal antibody (STJ23515) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EIF4A3 antibody (1-210) (STJ23515)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using EIF4B Rabbit pAb (STJ11107417) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EIF4B antibody (352-611 aa) (STJ11107417)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts of Rat skeletal muscle, using EIF4B antibody (STJ115264) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-EIF4B antibody (352-611) (STJ115264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of A549 cells, using eIF4B Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4B antibody (388-437 aa) (STJ92880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of EIF4B in paraffin-embedded human tonsil tissue using [KO Validated] EIF4B Rabbit polyclonal antibody (STJ11105552) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-EIF4B antibody (88-178) (STJ11105552)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of various lysates, using eIF4E antibody (STJ23516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-EIF4E antibody (1-100) (STJ23516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunofluorescence analysis of MCF7 cells using eIF4E antibody (STJ114889). Blue: DAPI for nuclear staining.

Anti-EIF4E antibody (1-217) (STJ114889)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse testis using 3 Mu g eIF4E antibody (STJ11101582). Western blot was performed from the immunoprecipitate using eIF4E (STJ11101582) at a dilution of 1:500.

Anti-EIF4E antibody (118-217) [S2MR] (STJ11101582)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various cells using eIF4E Polyclonal Antibody diluted at 1：1000

Anti-EIF4E antibody (168-217 aa) (STJ92881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis using eIF4E monoclonal antibody against HeLa (1) , HEK293 (2) and K562 (3) cell lysate.

Anti-EIF4E antibody [5D11] (STJ98016)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$194.40 - $469.80

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Immunofluorescence analysis of U-2 OS cells using EIF4E2 Rabbit polyclonal antibody (STJ26609) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-EIF4E2 antibody (1-240) (STJ26609)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-eIF4E3 antibody (1-90) (STJ500861)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

D.melanogaster

$577.13

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Anti-eIF4E3 antibody (1-90) {Biotin} (STJ500863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

D.melanogaster

$713.48

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Anti-eIF4E3 antibody (1-90) {FITC} (STJ500864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

D.melanogaster

$713.48

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Western blot analysis of 3T3 cells using eIF4E3 Polyclonal Antibody diluted at 1：1000

Anti-EIF4E3 antibody (141-190 aa) (STJ92882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-eIF4E3 antibody (150-240) (STJ500862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

D.melanogaster

$577.13

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Anti-eIF4E3 antibody (150-240) {Biotin} (STJ500865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

D.melanogaster

$713.48

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