• Immunofluorescence analysis of H9C2 cells using COPS8 Rabbit polyclonal antibody (STJ114618) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using COPS8 Rabbit polyclonal antibody (STJ114618) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using COPS8 Rabbit polyclonal antibody (STJ114618) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using COPS8 Rabbit polyclonal antibody (STJ114618) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-COPS8 antibody (1-209) (STJ114618)

SKU:
STJ114618

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COPS8 (1-209) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COPS8
Gene ID: 10920
Uniprot ID: CSN8_HUMAN
Immunogen Region: 1-209
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human COPS8 (NP_006701.1).
Immunogen Sequence: MPVAVMAESAFSFKKLLDQC ENQELEAPGGIATPPVYGQL LALYLLHNDMNNARYLWKRI PPAIKSANSELGGIWSVGQR IWQRDFPGIYTTINAHQWSE TVQPIMEALRDATRRRAFAL VSQAYTSIIADDFAAFVGLP VEEAVKGILEQGWQADSTTR MVLPRKPVAGALDVSFNKFI PLSEPAPVPPIPNEQQLARL TDYVAFLEN
Function Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Protein Name Cop9 Signalosome Complex Subunit 8
Sgn8
Signalosome Subunit 8
Cop9 Homolog
Hcop9
Jab1-Containing Signalosome Subunit 8
Database Links Reactome: R-HSA-5696394
Reactome: R-HSA-6781823
Reactome: R-HSA-8856825
Reactome: R-HSA-8951664
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Cop9 Signalosome Complex Subunit 8 antibody
Anti-Sgn8 antibody
Anti-Signalosome Subunit 8 antibody
Anti-Cop9 Homolog antibody
Anti-Hcop9 antibody
Anti-Jab1-Containing Signalosome Subunit 8 antibody
Anti-COPS8 antibody
Anti-CSN8 antibody

Information sourced from Uniprot.org

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