• Western blot analysis of the lysates from HepG2 cells using RFWD2 antibody.
  • Western blot analysis of lysates from LOVO cells, using RFWD2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-COP1 antibody (661-710 aa) (STJ92424)

SKU:
STJ92424

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase COP1 (661-710 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COP1
Gene ID: 64326
Uniprot ID: COP1_HUMAN
Immunogen Region: 661-710 aa
Specificity: COP1 Polyclonal Antibody detects endogenous levels of COP1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human RFWD2 at the amino acid range 661-710
Post Translational Modifications Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination.
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).
Protein Name E3 Ubiquitin-Protein Ligase Cop1
Constitutive Photomorphogenesis Protein 1 Homolog
Hcop1
Ring Finger And Wd Repeat Domain Protein 2
Ring Finger Protein 200
Ring-Type E3 Ubiquitin Transferase Rfwd2
Database Links Reactome: R-HSA-349425
Reactome: R-HSA-8951664
Cellular Localisation Nucleus Speckle
Cytoplasm
In The Nucleus
It Forms Nuclear Speckles
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Cop1 antibody
Anti-Constitutive Photomorphogenesis Protein 1 Homolog antibody
Anti-Hcop1 antibody
Anti-Ring Finger And Wd Repeat Domain Protein 2 antibody
Anti-Ring Finger Protein 200 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Rfwd2 antibody
Anti-COP1 antibody
Anti-RFWD2 antibody
Anti-RNF200 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance