Anti-COQ7 antibody (37-217) (STJ110079)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal COQ7 (37-217) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | COQ7 |
| Gene ID | 10229 |
| Uniprot ID | COQ7_HUMAN |
| Immunogen Region | 37-217 |
| Immunogen Sequence | SGMTLDNISRAAVDRIIRVD HAGEYGANRIYAGQMAVLGR TSVGPVIQKMWDQEKDHLKK FNELMVTFRVRPTVLMPLWN VLGFALGAGTALLGKEGAMA CTVAVEESIAHHYNNQIRTL MEEDPEKYEELLQLIKKFRD EELEHHDIGLDHDAELAPAY AVLKSIIQAGCRVAIYLSER L |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 37-217 of human COQ7 (NP_057222.2). |
Additional Info
| Tissue Specificity | Expressed dominantly in heart and skeletal muscle. |
| Function | Catalyzes the hydroxylation of the 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzoquinone at the C6 position and participates in the biosynthesis of ubiquinone (Probable). Catalyzes the reaction through a substrate-mediated reduction pathway, whereby NADH shuttles electrons to 5-methoxy-2-methyl-3-(all-trans-decaprenyl)benzoquinone, which then transfers the electrons to the two Fe(3+) centers. The binding of 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzoquinone (DMQn) mediates reduction of the diiron center by nicotinamide adenine dinucleotide (NADH) and initiates oxygen activation for subsequent DMQ hydroxylation. The physiological substrates are 5-methoxy-2-methyl-3-(all-trans-nonaprenyl)benzoquinone (DMQ(9)) and 5-methoxy-2-methyl-3-(all-trans-decaprenyl)benzoquinone (DMQ(10)), however in vitro the enzyme does not have any specificity concerning the length of the polyprenyl tail, and accepts tails of various lengths with similar efficiency. Also has a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides. Involved in lifespan determination in a ubiquinone-independent manner. Plays a role in modulating mitochondrial stress responses, acting in the nucleus, perhaps via regulating gene expression, independent of its characterized mitochondrial function in ubiquinone biosynthesis. |
| Protein Name | Nadph-Dependent 3-Demethoxyubiquinone 3-Hydroxylase - Mitochondrial3-Demethoxyubiquinone 3-HydroxylaseNadhTiming Protein Clk-1 HomologUbiquinone Biosynthesis Monooxygenase Coq7 |
| Database Links | Reactome: R-HSA-2142789 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix SideMitochondrionNucleusChromosome |
| Alternative Antibody Names | Anti-Nadph-Dependent 3-Demethoxyubiquinone 3-Hydroxylase - Mitochondrial antibodyAnti-3-Demethoxyubiquinone 3-Hydroxylase antibodyAnti-Nadh antibodyAnti-Timing Protein Clk-1 Homolog antibodyAnti-Ubiquinone Biosynthesis Monooxygenase Coq7 antibodyAnti-COQ7 antibody |
Information sourced from Uniprot.org