• Immunofluorescence analysis of U-2 OS cells using COPG2 Rabbit polyclonal antibody (STJ118261) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using COPG2 Rabbit polyclonal antibody (STJ118261) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of COPG2 in paraffin-embedded mouse brain using COPG2 Rabbit polyclonal antibody (STJ118261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of COPG2 in paraffin-embedded rat brain using COPG2 Rabbit polyclonal antibody (STJ118261) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using COPG2 Rabbit polyclonal antibody (STJ118261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COPG2 antibody (542-871) (STJ118261)

SKU:
STJ118261

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COPG2 (542-871) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COPG2
Gene ID: 26958
Uniprot ID: COPG2_HUMAN
Immunogen Region: 542-871
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 542-871 of human COPG2 (NP_036265.3).
Immunogen Sequence: LNATYIFNGLTVSVPGMEKA LHQYTLEPSEKPFDMKSIPL AMAPVFEQKAEITLVATKPE KLAPSRQDIFQEQLAAIPEF LNIGPLFKSSEPVQLTEAET EYFVRCIKHMFTNHIVFQFD CTNTLNDQLLEKVTVQMEPS DSYEVLSCIPAPSLPYNQPG ICYTLVRLPDDDPTAVAGSF SCTMKFTVRDCDPNTGVPDE DGYDDEYVLEDLEVTVSDHI QKVLKPNFAAAWEEVGDTF
Function The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins.the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
Protein Name Coatomer Subunit Gamma-2
Gamma-2-Coat Protein
Gamma-2-Cop
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Cytoplasm
Cytosol
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle
Copi-Coated Vesicle Membrane
The Coatomer Is Cytoplasmic Or Polymerized On The Cytoplasmic Side Of The Golgi
As Well As On The Vesicles/Buds Originating From It
Tends To Be More Abundant In The Trans-Golgi Network Compared To The Cis-Golgi
Alternative Antibody Names Anti-Coatomer Subunit Gamma-2 antibody
Anti-Gamma-2-Coat Protein antibody
Anti-Gamma-2-Cop antibody
Anti-COPG2 antibody

Information sourced from Uniprot.org

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