Primary Antibodies | St John's Laboratory

Chromatin immunoprecipitation analysis of extracts of K-562 cells, using TFAM antibody (STJ115513) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-TFAM antibody (100-200) (STJ115513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from HeLa cells using cGAS Rabbit pAb (STJ110633) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-MB21D1 antibody (350-522) (STJ110633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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IHC on rat spinal cord using Rabbit antibody to VPS45A (STJ13100462) at a concentration of 10 ug/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown) .

Anti-VPS45A antibody (STJ13100462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse

£331.50

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IF (confocal) on rat brain at a concentration of 30 ug/ml using Rabbit antibody to MAP2 (STJ13100172) , DAPI counterstained.

Anti-MAP2 antibody (350-400) (STJ13100172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse

£331.50

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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0. 01M containing Triton X 0. 005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to extracellular domain of human CD36 (Fatty acid translocase) : IgG (STJ13100092) diluted 1: 100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1: 100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Anti-CD36 antibody (ECD) (STJ13100092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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STJ73128 (0. 01µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIFM1 antibody (C-Term) (STJ73128)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ71828 (1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Thrombospondin antibody (N-Term) (STJ71828)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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STJ71692 (0. 5µg/ml) staining of Mouse Adipose lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Monoglyceride Lipase antibody (Internal) (STJ71692)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ70842 (0. 1µg/ml) staining of Human Liver (A) , (0. 03ug/ml) Testes (B) and Tonsil (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-GAPDH antibody (Internal) (STJ70842)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ70667 (0. 2µg/ml) staining of HumanThymus (A) and (0. 1ug/ml) Human Spleen (B) and Mouse Thymus © lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-MYD88 antibody (Internal) (STJ70667)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ70506 (0. 03µg/ml) staining of Human (A) Mouse (B) and Rat (C) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-VPS35/MEM3 antibody (C-Term) (STJ70506)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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STJ70381 (0. 1µg/ml) staining of HepG2 (A) and (0. 3ug/ml) MCF7 (B) nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-FOXA1/HNF3A antibody (C-Term) (STJ70381)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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STJ70052 (0. 05µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD20/MS4A1 antibody (C-Term) (STJ70052)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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STJ70014 (8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-SOCS1 antibody (C-Term) (STJ70014)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human/Mouse/Dog/Cow/Rat/Pig

£312.50

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Anti-CD5 antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-CD5 antibody (105-210aa) (STJ140164)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Anti-Rab3a antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-RAB3A antibody (181aa C-Term) (STJ140151)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Anti-ERBB1 antibody at 1:2500 dilution 50 µg of total protein per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-EGFR antibody (1162aa C-Term) (STJ140129)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Endogenous CDH1 detected with anti-CDH1 at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-FZR1 antibody (601 to 701) (STJ140099)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Endogenous CD31 detected at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PECAM1 antibody (690aa C-Term) (STJ140082)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Anti-Rab27 pan antibody at 1:500 dilution 50 µg of total protein per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-RAB27A antibody (120aa C-Term) (STJ140074)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/ELISA

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Anti-Rab5b antibody at 1:500 dilution 293 cells transfected with GFP-Rab5 lysates at 50 µg per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-RAB5B antibody (115aa C-Term) (STJ140061)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Endogenous CD45 detected with at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-PTPRC antibody (1260aa C-Term) (STJ140026)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Endogenous CD19 detected with at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-CD19 antibody (510aa C-Term) (STJ140024)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Endogenous CD4 detected at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-CD4 antibody (50-235aa ECD) (STJ140023)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Anti-CAV2 at 1:1000 dilution; 50 µg lysate per lane of transfected HEK293 and 100 µg of HaCat cell line; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-CAV2 antibody (50aa N-Term) (STJ140020)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot detection of EGFR in HCC827, A549 and MDA-MB-468 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.

Anti-EGFR antibody [3F12-1H7-A10] (STJ99228)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC/IP

Reactivity :

Human/Monkey

£144.00 - £348.00

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Western blot detection of Tubulin beta in COS7, LNCap, MCF7 and C2C12 cell lysates and using Tubulin beta mouse mAb (1:5000 diluted).Predicted band size: 55KDa.Observed band size: 55KDa.

Anti-TUBB antibody [1E1-E8-H4] (STJ99132)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Monkey/Goat/Hamster/Rat/Chlamydomonas

£144.00 - £348.00

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Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.

Anti-COX4I1 antibody [4D11-B3-E8] (STJ99044)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/ICC/IP/IHC

Reactivity :

Human/Mouse/Rat/Hamster/Goat/Monkey

£144.00 - £348.00

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Western blot analysis of 293T Hela lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-CDK6 antibody (280-325 aa) (STJ98721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 3T3 cells using Nanos Homologue 1 (NANOS1) Polyclonal Antibody diluted at 1:1500. Secondary antibody was diluted at 1:20000

Anti-NANOS1 antibody (151-200 aa) (STJ98619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of Hela cells using ETBR Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-EDNRB antibody (31-80 aa) (STJ98617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using MMP-9 monoclonal antibody against HEK293 (1) , MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.

Anti-MMP9 antibody [5G3] (STJ98248)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunofluorescence analysis of human-uterus tissue. 1, Desmin monoclonal antibody (1B12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-DES antibody [1B12] (STJ97531)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP8 antibody [2G12] (STJ97394)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HeLa, HepG2 cells using Acetyl-NF Kappa B-p65 (K314/K315) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-RELA-Lys314/Lys315 antibody (STJ97358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HeLa cells using Acetyl-Ub (K27) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-Ub-Lys27 antibody (STJ97356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of KB cells using CD244 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD244 antibody (101-150 aa) (STJ97310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-EIF4A1 antibody [M8] (STJ97038)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, BMP-2 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-RELA antibody (200-300 aa) [5G6] (STJ97013)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, CD15 monoclonal antibody (Q89) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-FUT4 antibody [Q89] (STJ97007)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, EFHD1 monoclonal antibody (3G2) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-EFHD1 antibody [3G2] (STJ96987)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, FH monoclonal antibody (7F1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-FH antibody [7F1] (STJ96975)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of human-uterus tissue. 1, CD10 monoclonal antibody (5B8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-MME antibody [5B8] (STJ96974)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, Aquaporin 4 monoclonal antibody (4H1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-AQP4 antibody [4H1] (STJ96964)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, CK16 monoclonal antibody (6F6) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-KRT16 antibody [6F6] (STJ96958)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of human-stomach tissue. 1, CD23 monoclonal antibody (1E9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-FCER2 antibody [1E9] (STJ96951)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HeLa cells using EMMPRIN Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-BSG antibody (221-270 aa) (STJ96836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of PC12 cells using FOXP3 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-FOXP3 antibody (381-430 aa) (STJ96811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

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