Anti-CD5 antibody (105-210aa) (STJ140164)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant peptide derived from within residues 105-210 aa of human CD5 produced in E. coli.
General Information
| Short Description | Goat polyclonal T-cell surface glycoprotein CD5 (105-210aa) antibody for use in WB, IHC-F and IHC-P in human, rat, mouse, canine and monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-F/IHC-P |
| Host | Goat |
| Reactivity | Human/Rat/Mouse/Canine/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 3 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:500-1:2000IHC-F 1:250-1:1000IHC-P 1:250-1:1000 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | CD5 |
| Gene ID | 921 |
| Uniprot ID | CD5_HUMAN |
| Accession Number | ENSG00000110448 |
| Immunogen | Recombinant peptide derived from within residues 105-210 aa of human CD5 produced in E. coli. |
| Immunogen Region | 105-210aa |
| Immunogen Sequence | QSSIICYGQLGSFSNCSHSR SSIICYGQLGSFSNCSHSRN SIICYGQLGSFSNCSHSRND IICYGQLGSFSNCSHSRNDM ICYGQLGSFSNCSHSRNDMC CYGQLGSFSNCSHSRNDMCH |
| Specificity | Detects endogenous levels of CD5 by Western blot. |
Additional Info
| Post Translational Modifications | Phosphorylated on serine, threonine and tyrosine residues following TCR stimulation. Phosphorylated by LCK on Tyr-453 and Tyr-487 upon TCR engagement. |
| Function | Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation. Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production. |
| Protein Name | T-Cell Surface Glycoprotein Cd5Lymphocyte Antigen T1/Leu-1Cd Antigen Cd5 |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd5 antibodyAnti-Lymphocyte Antigen T1/Leu-1 antibodyAnti-Cd Antigen Cd5 antibodyAnti-CD5 antibodyAnti-LEU1 antibody |
Information sourced from Uniprot.org