• Western blot analysis of lysates from 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Polyclonal Antibody (cat: (STJ97090) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
  • IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
  • Western blot analysis of 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue, diluted at 1:2000.
  • Immunofluorescence analysis of Mouse-brain tissue. 1, Aquaporin 4 monoclonal antibody (4H1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Human-appendix tissue. 1, Aquaporin 4 monoclonal antibody (4H1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, Aquaporin 4 monoclonal antibody (4H1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-AQP4 antibody [4H1] (STJ96964)

SKU:
STJ96964

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Aquaporin-4 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 4H1
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:1000
IF 1:100-200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AQP4
Gene ID: 361
Uniprot ID: AQP4_HUMAN
Specificity: The antibody detects endogenous Aquaporin 4 proteins.
Immunogen: Synthetic Peptide of Aquaporin 4
Post Translational Modifications Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40%. Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated.
Function Forms a water-specific channel. Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability.
Protein Name Aquaporin-4
Aqp-4
Mercurial-Insensitive Water Channel
Miwc
Wch4
Database Links Reactome: R-HSA-432040
Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Basolateral Cell Membrane
Endosome Membrane
Sarcolemma
Cell Projection
Activation Of The Vasopressin Receptor Avpr1a Triggers Aqp4 Phosphorylation At Ser-180 And Promotes Its Internalization From The Cell Membrane
Detected On Brain Astrocyte Processes And Astrocyte Endfeet Close To Capillaries
Alternative Antibody Names Anti-Aquaporin-4 antibody
Anti-Aqp-4 antibody
Anti-Mercurial-Insensitive Water Channel antibody
Anti-Miwc antibody
Anti-Wch4 antibody
Anti-AQP4 antibody

Information sourced from Uniprot.org

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